Glucose Transporter Glut1 Antibody (A-4): sc-377228

Glut1 Antibody (A-4) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 218-260 of human Glut1, also known as Glucose Transporter Type 1, SLC2A1, or HepG2 Glucose Transporter. Glut1 monoclonal antibody (A-4) is validated for applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Glut1 is a 12-pass transmembrane protein that facilitates the basal transport of glucose across plasma membranes, making Glut1 essential for cellular energy production and metabolism. Unlike insulin-regulated transporters like Glut4, Glut1 remains constitutively present at the plasma membrane and ensures glucose availability under varying conditions, including hypoxia. Glut1 is ubiquitously expressed but appears especially abundant in tissues with high glucose demands, such as the brain, red blood cells, and endothelial cells of the blood-brain barrier. Glut1 maintains glucose homeostasis, and Glut1 dysregulation has been associated with various diseases, including cancer and Glut1 Deficiency Syndrome. Understanding Glut1 function remains critical for studying metabolic pathways, energy regulation, and cellular adaptation to stress. Anti-Glut1 antibody (A-4) serves as an effective tool for investigating Glut1′s role in physiological and pathological processes.

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Glucose Transporter Glut1 Antibody (A-4) References:

1. Hemiplegic Migraine in Glut1 Deficiency Syndrome and Paroxysmal Dyskinesia at Ketogenic Diet Induction: Case Report and Literature Review.  |  Gburek-Augustat, J., et al. 2020. Mov Disord Clin Pract. 7: 965-970. PMID: 33163569
2. The Effect of GLUT1 on the Survival Rate and Immune Cell Infiltration of Lung Adenocarcinoma and Squamous Cell Carcinoma: A Meta and Bioinformatics Analysis.  |  Zhang, G., et al. 2022. Anticancer Agents Med Chem. 22: 223-238. PMID: 34238200
3. Glut1 deficiency syndrome: New and emerging insights into a prototypical brain energy failure disorder.  |  Tang, M. and Monani, UR. 2021. Neurosci Insights. 16: 26331055211011507. PMID: 34589708
4. Glucose Transporter Glut1-Dependent Metabolic Reprogramming Regulates Lipopolysaccharide-Induced Inflammation in RAW264.7 Macrophages.  |  Cornwell, A., et al. 2023. Biomolecules. 13: PMID: 37238640
5. GLUT1 promotes cell proliferation via binds and stabilizes phosphorylated EGFR in lung adenocarcinoma.  |  Zhou, Z., et al. 2024. Cell Commun Signal. 22: 303. PMID: 38831321
6. Molecular regulation of GLUT-4 targeting in 3T3-L1 adipocytes.  |  Marsh, BJ., et al. 1995. J Cell Biol. 130: 1081-91. PMID: 7544796
7. Regulation of glucose transporters in cultured rat adipocytes: synergistic effect of insulin and dexamethasone on GLUT4 gene expression through promoter activation.  |  Hajduch, E., et al. 1995. Endocrinology. 136: 4782-9. PMID: 7588207
8. Cardiac and adipose tissue abnormalities but not diabetes in mice deficient in GLUT4.  |  Katz, EB., et al. 1995. Nature. 377: 151-5. PMID: 7675081
9. Insulin-sensitive targeting of the GLUT4 glucose transporter in L6 myoblasts is conferred by its COOH-terminal cytoplasmic tail.  |  Haney, PM., et al. 1995. J Cell Biol. 129: 641-58. PMID: 7730401
10. Expression and compartmentalization of caveolin in adipose cells: coordinate regulation with and structural segregation from GLUT4.  |  Kandror, KV., et al. 1995. J Cell Biol. 129: 999-1006. PMID: 7744970
11. Hypothalamic GLUT 4 expression: a glucose- and insulin-sensing mechanism?  |  Livingstone, C., et al. 1995. Mol Cell Endocrinol. 107: 67-70. PMID: 7796936
12. Evidence that facilitative glucose transporters may fold as beta-barrels.  |  Fischbarg, J., et al. 1993. Proc Natl Acad Sci U S A. 90: 11658-62. PMID: 8265604

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Glucose Transporter Glut1 Antibody (A-4) has 63 citations in a variety of scientific publications.

Primary antibodies like Anti-Glucose Transporter Glut1 Antibody (A-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Glucose Transporter Glut1 Antibody (A-4) Specifications

Alternate Product Name: Glut1

Product Clone Name: A-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 218-260

Epitope Target: Glut1

Epitope Species: human

Product Reactivity/Detection: Glut1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 63 citations in various scientific publications

Available Conjugates/Forms for Glucose Transporter Glut1 Antibody (A-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin