Date published: 2025-9-5
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Glucose Transporter Glut1 Antibody (A-4) - Immunohistochemistry - Image 359497
Glut1 抗体 (A-4): sc-377228. 免疫过氧化物酶检测福尔马林固定, 石蜡包埋的人类胎盘组织中的 Glut1, 显示滋养细胞膜染色. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
Glut1 抗体 (A-4): sc-377228. 免疫过氧化物酶检测福尔马林固定, 石蜡包埋的人类胎盘组织中的 Glut1, 显示滋养细胞膜染色. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

Glucose Transporter Glut1 抗体 (A-4): sc-377228

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说明书
  • Glucose Transporter Glut1 抗体 A-4 是小鼠单克隆 IgG1 κ，Glucose Transporter Glut1抗体, 在63篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Glut1的氨基酸218-260
  • Glucose Transporter Glut1 抗体 (A-4) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 Glut1
  • 抗Glucose Transporter Glut1抗体（A-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Glucose Transporter Glut1 (A-4): sc-377228的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Glucose Transporter Glut1 抗体 (A-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Glucose Transporter Glut1 抗体 (A-4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Glut 抗体 包括 Glut1, Glut10, Glut2, Glut3, Glut4, Glut5和Glut6

Glut1抗体（A-4）是一种IgG1 κ小鼠单克隆Glut1抗体（也被称作葡萄糖转运蛋白GLUT1抗体、SLC2A1抗体或HepG2葡萄糖转运蛋白抗体），可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC(P)）和酶联免疫吸附测定（ELISA）检测到人源的Glut1蛋白。非偶联形式的抗Glut1抗体以及多种偶联形式的抗Glut1抗体（包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物）均可作为Glut1抗体（A-4）使用。葡萄糖对于哺乳动物细胞的代谢至关重要。其穿过细胞膜的过程由一系列被称为葡萄糖转运蛋白或Gluts的转运蛋白介导。在脂肪和肌肉组织中，胰岛素刺激葡萄糖摄取量迅速且显著增加，这在很大程度上是由于胰岛素可诱导的葡萄糖转运蛋白Glut4的重新分布。响应胰岛素后，Glut4迅速从细胞内储存位点转移到质膜，在那里与葡萄糖结合。相比之下，普遍表达的葡萄糖转运蛋白Glut1则持续靶向质膜，并在响应胰岛素时表现出不那么显著的转位。Glut1和Glut4是十二次跨膜蛋白（12TM），其羧基末端可能决定它们的细胞定位。Glut4的异常表达被认为与肥胖和糖尿病等疾病有关。Glut4基因敲除小鼠的研究表明，虽然维持正常葡萄糖水平不需要功能性Glut4蛋白，但Glut4蛋白对于持续生长、正常细胞葡萄糖、脂肪代谢和延长寿命是必要的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Glucose Transporter Glut1 抗体 (A-4) 参考文献:

  1. 半身偏瘫性偏头痛与生酮饮食诱导的阵发性运动障碍：病例报告和文献综述。  |  Gburek-Augustat, J., et al. 2020. Mov Disord Clin Pract. 7: 965-970. PMID: 33163569
  2. GLUT1对肺腺癌和鳞状细胞癌的存活率和免疫细胞浸润的影响：荟萃分析和生物信息学分析。  |  Zhang, G., et al. 2022. Anticancer Agents Med Chem. 22: 223-238. PMID: 34238200
  3. Glut1缺乏综合征：对典型脑能量衰竭疾病的新见解。  |  Tang, M. and Monani, UR. 2021. Neurosci Insights. 16: 26331055211011507. PMID: 34589708
  4. 葡萄糖转运蛋白Glut1依赖性代谢重编程调节RAW264.7巨噬细胞中脂多糖诱导的炎症。  |  Cornwell, A., et al. 2023. Biomolecules. 13: PMID: 37238640
  5. GLUT1通过结合和稳定磷酸化EGFR促进肺腺癌细胞增殖。  |  Zhou, Z., et al. 2024. Cell Commun Signal. 22: 303. PMID: 38831321
  6. 3T3-L1 脂肪细胞中 GLUT-4 靶向的分子调控。  |  Marsh, BJ., et al. 1995. J Cell Biol. 130: 1081-91. PMID: 7544796
  7. 培养的大鼠脂肪细胞中葡萄糖转运体的调控：胰岛素和地塞米松通过启动子激活对 GLUT4 基因表达的协同作用。  |  Hajduch, E., et al. 1995. Endocrinology. 136: 4782-9. PMID: 7588207
  8. 缺乏 GLUT4 的小鼠会出现心脏和脂肪组织异常，但不会患糖尿病。  |  Katz, EB., et al. 1995. Nature. 377: 151-5. PMID: 7675081
  9. 葡萄糖转运体 GLUT4 在 L6 肌母细胞中的胰岛素敏感靶向是由其 COOH 端细胞质尾部决定的。  |  Haney, PM., et al. 1995. J Cell Biol. 129: 641-58. PMID: 7730401
  10. 洞穴素在脂肪细胞中的表达和分区：与 GLUT4 的协调调控和结构分离。  |  Kandror, KV., et al. 1995. J Cell Biol. 129: 999-1006. PMID: 7744970
  11. 下丘脑 GLUT 4 的表达：葡萄糖和胰岛素感应机制？  |  Livingstone, C., et al. 1995. Mol Cell Endocrinol. 107: 67-70. PMID: 7796936
  12. 有证据表明，促进性葡萄糖转运体可能折叠成β-桶。  |  Fischbarg, J., et al. 1993. Proc Natl Acad Sci U S A. 90: 11658-62. PMID: 8265604

订购信息

产品名称产品编号规格价格数量收藏夹

Glucose Transporter Glut1 抗体 (A-4)

sc-377228
200 µg/ml
$316.00

Glucose Transporter Glut1 (A-4): m-IgG Fc BP-HRP 套装

sc-529991
200 µg Ab; 10 µg BP
$354.00

Glucose Transporter Glut1 (A-4): m-IgGκ BP-HRP 套装

sc-523241
200 µg Ab, 40 µg BP
$354.00

Glucose Transporter Glut1 (A-4): m-IgG1 BP-HRP 套装

sc-543806
200 µg Ab; 20 µg BP
$354.00

Glucose Transporter Glut1 抗体 (A-4) AC

sc-377228 AC
500 µg/ml, 25% agarose
$416.00

Glucose Transporter Glut1 抗体 (A-4) HRP

sc-377228 HRP
200 µg/ml
$316.00

Glucose Transporter Glut1 抗体 (A-4) FITC

sc-377228 FITC
200 µg/ml
$330.00

Glucose Transporter Glut1 抗体 (A-4) PE

sc-377228 PE
200 µg/ml
$343.00

Glucose Transporter Glut1 抗体 (A-4) Alexa Fluor® 488

sc-377228 AF488
200 µg/ml
$357.00

Glucose Transporter Glut1 抗体 (A-4) Alexa Fluor® 546

sc-377228 AF546
200 µg/ml
$357.00

Glucose Transporter Glut1 抗体 (A-4) Alexa Fluor® 594

sc-377228 AF594
200 µg/ml
$357.00

Glucose Transporter Glut1 抗体 (A-4) Alexa Fluor® 647

sc-377228 AF647
200 µg/ml
$357.00

Glucose Transporter Glut1 抗体 (A-4) Alexa Fluor® 680

sc-377228 AF680
200 µg/ml
$357.00

Glucose Transporter Glut1 抗体 (A-4) Alexa Fluor® 790

sc-377228 AF790
200 µg/ml
$357.00

hola, me podrias enviar una muestra gratis de Glucose Transporter Glut1 Anticuerpo (A-4): sc-377228?

Asked by: juli1973
Thank you for your question. Please contact our technical service team directly to make the necessary arrangements.
Answered by: Tech Support Europe
Date published: 2023-01-26

Hi, this product detect GLUT-1 in mouse?

Asked by: bobbym
Thank you for your question. Glucose Transporter Glut1 Antibody (A-4): sc-377228 is only recommended for detection of Glut1 of human origin by WB, IP, IF, IHC(P) and ELISA. We do not recommend it for use in murine cell or tissue samples.
Answered by: Tech Support Europe
Date published: 2024-07-02

What are possible reasons for the smear-band appearance after probing western blots with this antibody? Could you provide the details for the electrophoretic and transfer conditions of the representative positive controls for western blot? Thank you

Asked by: DP UoP
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-04-07

I work with porcine brain capillary endothelial cells. Has the antibody been tested in pig or porcine cells?

Asked by: joshua
Thank you for your question. Glut1 Antibody (A-4): sc-377228 has been tested in cells and tissues from human, rat, and mouse and is only recommended for detection of Glut1 of human origin by WB, IP, IF, IHC(P) and ELISA.
Answered by: Technical Support Europe
Date published: 2020-10-21

What type of secondary antibodies are recommended to use for this antibody? Is it possible to use horseradish peroxidase secondary antibodies?

Asked by: Lea_
Thank you for your question. You can use any anti-mouse secondary, including HRP conjugated secondary antibodies. We recommend using our Mouse IgG Binding protein, you can find it here: https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: Tech Service
Date published: 2019-01-16

For immunoprecipitation, does the antibody detect dentaured or native protein?

Asked by: orangina
Thank you for your question. Glut1 Antibody (A-4): sc-377228 is recommended for detection of Glut1 of human origin by WB, IP, IF, IHC(P) and ELISA, so it detects both the denatured and the native protein.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-29

Hi, This product detect GLUT-1 in Mouse?

Asked by: Rigoberto
Thank you for your question. We do not currently recommend this antibody, sc-377228: Glut1 (A-4), for use in mouse samples because we have not yet tested it in mouse. The antibody is raised against a recombinant protein sequence that shares significant (95%) identity with the mouse Glut1 protein. Therefore, it is highly likely that the antibody will cross-react with mouse Glut1. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to request a free sample to test in mouse. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-12-14

Can Glut1 Antibody (A-4): sc-377228 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, Glut1 Antibody (A-4): sc-377228 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 6
Date published: 2017-01-09
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Rated 4 out of 5 by from Antibody works well on Westerns and IFUsing a 1:200 dilution, I easily visualized the GLUT1 protein after cell staining and microscopy, as well as on Western blots (after solubilizing the cell membranes) to extract membrane proteins. I recommend this product.
Date published: 2022-06-09
Rated 4 out of 5 by from Good productThe antibody works well for IHC staining performed on murine paraffin-embedded tissues. The signal was very specific and clear, no background signal was detected. Highly recommended.
Date published: 2021-07-14
Rated 5 out of 5 by from well stainWhen I stained the cerebral infarction with this antibody, it stained the vascular endothelial cells very well.
Date published: 2020-04-05
Rated 4 out of 5 by from Works fine for WBIt works fine for WB. Not recommended for flow cytometry.
Date published: 2020-02-18
Rated 1 out of 5 by from Did not work for WBDid not see any bands for western blots for DLD-1 cell lines. I tried 1:100 and 1:500 (with 1:10,000 and 1:5000 secondary).
Date published: 2019-07-17
Rated 1 out of 5 by from kein Ergebnis beim WBHat beim WB (1/200) mit SHSY5Y und HEP Zellen keine spezifische Bande gezeigt
Date published: 2018-04-03
Rated 4 out of 5 by from WBI got the bands and correct position by wextern blot (1：200), but hope work better.
Date published: 2018-03-01
Rated 5 out of 5 by from Very goodWorks very well in human neural stem cells. Visible band at 55 kDa.
Date published: 2017-12-14
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Glucose Transporter Glut1 抗体 (A-4) is rated 4.3 out of 5 by 28.
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