GlnRS Antibody (C-1): sc-271078

GlnRS Antibody (C-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects GlnRS protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). GlnRS Antibody (C-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Glutaminyl-tRNA synthetase (GlnRS), also known as QARS, is a crucial enzyme composed of 775 amino acids that belongs to the class-I aminoacyl-tRNA synthetase family. GlnRS is primarily located in the cytoplasm and plays a vital role in protein synthesis by catalyzing the attachment of L-glutamine to its corresponding tRNA, forming Gln-tRNA(Gln) in an ATP-dependent manner. GlnRS functions extend beyond amino acid activation as part of a multi-protein complex that includes nine different aminoacyl-tRNA synthetases, each specific for a distinct amino acid, ensuring protein translation fidelity. In eukaryotic cells, GlnRS is essential for synthesizing Gln-tRNA(Gln), while in prokaryotes and organelles like mitochondria and chloroplasts, the process involves a more complex two-step mechanism including misacylation and amidation reactions. GlnRS′s ability to accurately charge tRNA with glutamine is critical for maintaining cellular function and responding to metabolic demands, making GlnRS a significant target for research in various biological and disease contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

GlnRS Antibody (C-1) References:

1. Characterization of the human laminin beta2 chain locus (LAMB2): linkage to a gene containing a nonprocessed, transcribed LAMB2-like pseudogene (LAMB2L) and to the gene encoding glutaminyl tRNA synthetase (QARS).  |  Durkin, ME., et al. 1999. Cytogenet Cell Genet. 84: 173-8. PMID: 10393422
2. Nucleolar localization of human methionyl-tRNA synthetase and its role in ribosomal RNA synthesis.  |  Ko, YG., et al. 2000. J Cell Biol. 149: 567-74. PMID: 10791971
3. Catalytic peptide of human glutaminyl-tRNA synthetase is essential for its assembly to the aminoacyl-tRNA synthetase complex.  |  Kim, T., et al. 2000. J Biol Chem. 275: 21768-72. PMID: 10801842
4. Heat shock protein 90 mediates protein-protein interactions between human aminoacyl-tRNA synthetases.  |  Kang, J., et al. 2000. J Biol Chem. 275: 31682-8. PMID: 10913161
5. Glutamine-dependent antiapoptotic interaction of human glutaminyl-tRNA synthetase with apoptosis signal-regulating kinase 1.  |  Ko, YG., et al. 2001. J Biol Chem. 276: 6030-6. PMID: 11096076
6. Mutant enzymes and tRNAs as probes of the glutaminyl-tRNA synthetase: tRNA(Gln) interaction.  |  Englisch-Peters, S., et al. 1991. Biochimie. 73: 1501-8. PMID: 1725262
7. Purification and functional characterization of the Glu-tRNA(Gln) amidotransferase from Chlamydomonas reinhardtii.  |  Jahn, D., et al. 1990. J Biol Chem. 265: 8059-64. PMID: 1970821
8. Evolution of the Glx-tRNA synthetase family: the glutaminyl enzyme as a case of horizontal gene transfer.  |  Lamour, V., et al. 1994. Proc Natl Acad Sci U S A. 91: 8670-4. PMID: 8078941
9. Recognition of tRNAs by aminoacyl-tRNA synthetases.  |  Cavarelli, J. and Moras, D. 1993. FASEB J. 7: 79-86. PMID: 8422978
10. Cloning and sequencing of the first plant GlnRS and GluRS genes.  |  Siatecka, M., et al. 1995. Nucleic Acids Symp Ser. 160-2. PMID: 8643358
11. Glutaminyl-tRNA synthetase.  |  Freist, W., et al. 1997. Biol Chem. 378: 1103-17. PMID: 9372179
12. Macromolecular assemblage of aminoacyl-tRNA synthetases: identification of protein-protein interactions and characterization of a core protein.  |  Quevillon, S., et al. 1999. J Mol Biol. 285: 183-95. PMID: 9878398

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GlnRS Antibody (C-1) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-GlnRS Antibody (C-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GlnRS Antibody (C-1) Specifications

Alternate Product Name: GlnRS

Product Clone Name: C-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 513-775

Epitope Target: GlnRS

Epitope Species: human

Product Reactivity/Detection: GlnRS

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for GlnRS Antibody (C-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin