GLK Antibody (C-6): sc-515064
- GLK Antibody (C-6) is a mouse monoclonal IgG2b κ GLK antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 862-885 at the C-terminus of GLK of human origin
- GLK Antibody (C-6) is recommended for detection of GLK and KHS of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-GLK Antibody (C-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GLK Antibody (C-6) for WB applications. These reagents are now offered in bundles with GLK Antibody (C-6) (see ordering information below).
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SEE ALSO...
GLK Antibody (C-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects GLK protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-GLK antibody (C-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. GLK protein, also known as GC-like kinase, is an 885 amino acid serine/threonine kinase that plays a crucial role in cellular signaling pathways, particularly in activating the c-Jun N-terminal kinase (JNK) pathway. This pathway is essential for various cellular processes, including stress responses, apoptosis, and differentiation, making GLK a significant player in regulating cellular functions. GLK functions upstream of MEKK1 in the JNK signaling cascade, indicating importance in mediating signals from G-protein coupled receptors to downstream MAP kinase pathways. Understanding GLK and its interactions with other kinases can provide valuable insights into signal transduction mechanisms and potential implications in various diseases, including cancer and neurodegenerative disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GLK Antibody (C-6) References:
- The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein beta gamma subunits to downstream signalling components. | Leberer, E., et al. 1992. EMBO J. 11: 4815-24. PMID: 1464311
- Molecular characterization of Ste20p, a potential mitogen-activated protein or extracellular signal-regulated kinase kinase (MEK) kinase kinase from Saccharomyces cerevisiae. | Wu, C., et al. 1995. J Biol Chem. 270: 15984-92. PMID: 7608157
- Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade. | Hu, MC., et al. 1996. Genes Dev. 10: 2251-64. PMID: 8824585
- NIK is a new Ste20-related kinase that binds NCK and MEKK1 and activates the SAPK/JNK cascade via a conserved regulatory domain. | Su, YC., et al. 1997. EMBO J. 16: 1279-90. PMID: 9135144
- Activation of the c-Jun N-terminal kinase pathway by a novel protein kinase related to human germinal center kinase. | Diener, K., et al. 1997. Proc Natl Acad Sci U S A. 94: 9687-92. PMID: 9275185
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GLK Antibody (C-6) | sc-515064 | 200 µg/ml | $322.00 | |||
GLK Antibody (C-6): m-IgG Fc BP-HRP Bundle | sc-531279 | 200 µg Ab; 10 µg BP | $361.00 | |||
GLK Antibody (C-6): m-IgGκ BP-HRP Bundle | sc-524923 | 200 µg Ab, 40 µg BP | $361.00 | |||
GLK Antibody (C-6): m-IgG2b BP-HRP Bundle | sc-550275 | 200 µg Ab; 10 µg BP | $361.00 | |||
GLK Antibody (C-6) AC | sc-515064 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GLK Antibody (C-6) HRP | sc-515064 HRP | 200 µg/ml | $322.00 | |||
GLK Antibody (C-6) FITC | sc-515064 FITC | 200 µg/ml | $336.00 | |||
GLK Antibody (C-6) PE | sc-515064 PE | 200 µg/ml | $349.00 | |||
GLK Antibody (C-6) Alexa Fluor® 488 | sc-515064 AF488 | 200 µg/ml | $364.00 | |||
GLK Antibody (C-6) Alexa Fluor® 546 | sc-515064 AF546 | 200 µg/ml | $364.00 | |||
GLK Antibody (C-6) Alexa Fluor® 594 | sc-515064 AF594 | 200 µg/ml | $364.00 | |||
GLK Antibody (C-6) Alexa Fluor® 647 | sc-515064 AF647 | 200 µg/ml | $364.00 | |||
GLK Antibody (C-6) Alexa Fluor® 680 | sc-515064 AF680 | 200 µg/ml | $364.00 | |||
GLK Antibody (C-6) Alexa Fluor® 790 | sc-515064 AF790 | 200 µg/ml | $364.00 | |||
GLK (C-6) Neutralizing Peptide | sc-515064 P | 100 µg/0.5 ml | $69.00 |