GLIS1 Antibody (A-3): sc-373755
- GLIS1 Antibody (A-3) is a mouse monoclonal IgG1 κ GLIS1 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 582-613 within an internal region of GLIS1 of mouse origin
- GLIS1 Antibody (A-3) is recommended for detection of GLIS1 of mouse and rat origin by WB, IP, IF and ELISA
- Anti-GLIS1 Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-373755 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GLIS1 Antibody (A-3) for WB applications. These reagents are now offered in bundles with GLIS1 Antibody (A-3) (see ordering information below).
QUICK LINKS
GLIS1 Antibody (A-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects GLIS1 protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GLIS1 (A-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GLIS1 protein, consisting of 789 amino acids, is encoded by the mouse gene Glis1 and is primarily located in the nucleus, where GLIS1 plays a crucial role as both a transcriptional repressor and activator. This dual functionality is vital for regulating gene expression during development, particularly in mesodermal structures such as craniofacial regions, branchial arches, somites, vibrissal and hair follicles, limb buds, and myotomes. GLIS1 belongs to the GLI C2H2-type zinc-finger protein family and features five C2H2-type zinc fingers, which are essential for DNA interaction and protein binding. GLIS1 binds to the consensus sequence 5′-GACCACCCAC-3′, influencing the Sonic hedgehog (Shh)-patched signaling pathway, which is critical for proper embryonic development. Understanding GLIS1 regulation and associated pathways is important for elucidating mechanisms behind human birth defects and various cancers, making anti-GLIS1 antibody (A-3) a valuable tool for researchers in developmental biology and oncology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GLIS1 Antibody (A-3) | sc-373755 | 200 µg/ml | $316.00 | |||
GLIS1 Antibody (A-3): m-IgG Fc BP-HRP Bundle | sc-529550 | 200 µg Ab; 10 µg BP | $354.00 | |||
GLIS1 Antibody (A-3): m-IgGκ BP-HRP Bundle | sc-522483 | 200 µg Ab, 40 µg BP | $354.00 | |||
GLIS1 Antibody (A-3): m-IgG1 BP-HRP Bundle | sc-543575 | 200 µg Ab; 20 µg BP | $354.00 | |||
GLIS1 Antibody (A-3) X | sc-373755 X | 200 µg/0.1 ml | $316.00 | |||
GLIS1 Antibody (A-3) AC | sc-373755 AC | 500 µg/ml, 25% agarose | $416.00 | |||
GLIS1 Antibody (A-3) HRP | sc-373755 HRP | 200 µg/ml | $316.00 | |||
GLIS1 Antibody (A-3) FITC | sc-373755 FITC | 200 µg/ml | $330.00 | |||
GLIS1 Antibody (A-3) PE | sc-373755 PE | 200 µg/ml | $343.00 | |||
GLIS1 Antibody (A-3) Alexa Fluor® 488 | sc-373755 AF488 | 200 µg/ml | $357.00 | |||
GLIS1 Antibody (A-3) Alexa Fluor® 546 | sc-373755 AF546 | 200 µg/ml | $357.00 | |||
GLIS1 Antibody (A-3) Alexa Fluor® 594 | sc-373755 AF594 | 200 µg/ml | $357.00 | |||
GLIS1 Antibody (A-3) Alexa Fluor® 647 | sc-373755 AF647 | 200 µg/ml | $357.00 | |||
GLIS1 Antibody (A-3) Alexa Fluor® 680 | sc-373755 AF680 | 200 µg/ml | $357.00 | |||
GLIS1 Antibody (A-3) Alexa Fluor® 790 | sc-373755 AF790 | 200 µg/ml | $357.00 | |||
GLIS1 (A-3) Neutralizing Peptide | sc-373755 P | 100 µg/0.5 ml | $68.00 |