GLIS1 Antibody (E-6): sc-365857
- GLIS1 Antibody (E-6) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 532-567 near the C-terminus of GLIS1 of human origin
- GLIS1 Antibody (E-6) is recommended for detection of GLIS1 of human origin by WB, IP, IF and ELISA
- Anti-GLIS1 Antibody (E-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-365857 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GLIS1 Antibody (E-6) for WB applications. These reagents are now offered in bundles with GLIS1 Antibody (E-6) (see ordering information below).
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GLIS1 Antibody (E-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects GLIS1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GLIS1 Antibody (E-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GLIS1 is a 789 amino acid protein encoded by the human gene GLIS1, primarily located in the nucleus, where GLIS1 plays a crucial role as both a transcriptional repressor and activator. This dual functionality is vital for regulating gene expression during development, particularly in mesodermal structures such as craniofacial regions, branchial arches, and limb buds. GLIS1 belongs to the GLI C2H2-type zinc-finger protein family and features five C2H2-type zinc fingers, which are essential for DNA-binding capabilities. GLIS1 binds to the consensus sequence 5′-GACCACCCAC-3′, influencing the transcription of target genes involved in critical developmental pathways. Understanding GLIS1 regulation is significant, as such knowledge may provide insights into the mechanisms underlying human birth defects and various cancers, highlighting GLIS1′s importance in developmental biology and disease research.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GLIS1 Antibody (E-6) References:
- Genomic structure of the gene encoding the human GLI-related, Krüppel-like zinc finger protein GLIS2. | Zhang, F. and Jetten, AM. 2001. Gene. 280: 49-57. PMID: 11738817
- Characterization of Glis2, a novel gene encoding a Gli-related, Krüppel-like transcription factor with transactivation and repressor functions. Roles in kidney development and neurogenesis. | Zhang, F., et al. 2002. J Biol Chem. 277: 10139-49. PMID: 11741991
- Identification of Glis1, a novel Gli-related, Kruppel-like zinc finger protein containing transactivation and repressor functions. | Kim, YS., et al. 2002. J Biol Chem. 277: 30901-13. PMID: 12042312
- A novel gene, GliH1, with homology to the Gli zinc finger domain not required for mouse development. | Nakashima, M., et al. 2002. Mech Dev. 119: 21-34. PMID: 12385751
- GLIS3, a novel member of the GLIS subfamily of Krüppel-like zinc finger proteins with repressor and activation functions. | Kim, YS., et al. 2003. Nucleic Acids Res. 31: 5513-25. PMID: 14500813
- Regulatory role for Krüppel-like zinc-finger protein Gli-similar 1 (Glis1) in PMA-treated and psoriatic epidermis. | Nakanishi, G., et al. 2006. J Invest Dermatol. 126: 49-60. PMID: 16417217
- Characterization of the promoter region and genomic organization of GLI, a member of the Sonic hedgehog-Patched signaling pathway. | Liu, CZ., et al. 1998. Gene. 209: 1-11. PMID: 9524201
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GLIS1 Antibody (E-6) | sc-365857 | 200 µg/ml | $316.00 | |||
GLIS1 Antibody (E-6): m-IgG Fc BP-HRP Bundle | sc-529494 | 200 µg Ab; 10 µg BP | $354.00 | |||
GLIS1 Antibody (E-6): m-IgGκ BP-HRP Bundle | sc-522397 | 200 µg Ab, 40 µg BP | $354.00 | |||
GLIS1 Antibody (E-6): m-IgG2a BP-HRP Bundle | sc-547320 | 200 µg Ab; 10 µg BP | $354.00 | |||
GLIS1 Antibody (E-6) X | sc-365857 X | 200 µg/0.1 ml | $316.00 | |||
GLIS1 Antibody (E-6) AC | sc-365857 AC | 500 µg/ml, 25% agarose | $416.00 | |||
GLIS1 Antibody (E-6) HRP | sc-365857 HRP | 200 µg/ml | $316.00 | |||
GLIS1 Antibody (E-6) FITC | sc-365857 FITC | 200 µg/ml | $330.00 | |||
GLIS1 Antibody (E-6) PE | sc-365857 PE | 200 µg/ml | $343.00 | |||
GLIS1 Antibody (E-6) Alexa Fluor® 488 | sc-365857 AF488 | 200 µg/ml | $357.00 | |||
GLIS1 Antibody (E-6) Alexa Fluor® 546 | sc-365857 AF546 | 200 µg/ml | $357.00 | |||
GLIS1 Antibody (E-6) Alexa Fluor® 594 | sc-365857 AF594 | 200 µg/ml | $357.00 | |||
GLIS1 Antibody (E-6) Alexa Fluor® 647 | sc-365857 AF647 | 200 µg/ml | $357.00 | |||
GLIS1 Antibody (E-6) Alexa Fluor® 680 | sc-365857 AF680 | 200 µg/ml | $357.00 | |||
GLIS1 Antibody (E-6) Alexa Fluor® 790 | sc-365857 AF790 | 200 µg/ml | $357.00 | |||
GLIS1 (E-6) Neutralizing Peptide | sc-365857 P | 100 µg/0.5 ml | $68.00 |