GKLF Antibody (B-8) recommended for detection of GKLF of mouse, rat and human origin by WB, IP, IF and ELISA

GKLF Antibody (B-8): sc-393462

GKLF Antibody (B-8) is rated 5.0 out of 5 by 4.
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Datasheets

    • Anti-GKLF Antibody (B-8) is a mouse monoclonal IgG2b (kappa light chain) GKLF antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 20-41 near the N-terminus of GKLF of mouse origin
    • Anti-GKLF Antibody (B-8) is recommended for detection of GKLF of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-GKLF Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-393462 X, 200 µg/0.1 ml)
    • blocking peptide, sc-393462 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GKLF (B-8): sc-393462.

Can GKLF (B-8): sc-393462 be frozen?

Asked by: jenni
No. The antibody solution contains gelatin. When the gelatin is frozen the antibody precipitates out of solution. However, if an antibody’s datasheet says that the antibody should be stored frozen, then the antibody can be frozen, as these antibodies do not contain gelatin.
Answered by: Technical Support 7
Date published: 2017-01-24
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Rated 5 out of 5 by from Works well by WB on mouse colon extracts This ab worked well by WB on mouse colon extracts (20ug) at a 1/500 dilution. Showed a signal a the right molecular weight after saturation and incubation of the ab in TBST-5% non fat milk and revelation with regular ECL
Date published: 2017-10-27
Rated 5 out of 5 by from Robust signal in WB! This GKLF monoclonal antibody gives a robust and consistent signal in mouse, rat and human whole cell lysates.
Date published: 2016-12-23
Rated 5 out of 5 by from Nice Western Blot data of GKLF expression in non Nice Western Blot data of GKLF expression in non-transfected and human GKLF transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-04-03
Rated 5 out of 5 by from Produced positive Western Blot data of GKLF Produced positive Western Blot data of GKLF expression in non-transfected 293T, mouse GKLF transfected 293T and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2013-09-30
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