GKLF/KLF4 Antibody (B-6): sc-166229
- GKLF/KLF4 Antibody (B-6) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1-180 of GKLF of human origin
- recommended for detection of GKLF of human origin by WB, IP, IF and ELISA
- TransCruz reagent for ChIP application (sc-166229 X, 200 µg/0.1 ml)
- See GKLF/EKLF/LKLF/KLF4/1/2 (F-8): sc-166238 for GKLF/KLF4 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for GKLF/KLF4 Antibody (B-6) for WB applications. These reagents are now offered in bundles with GKLF/KLF4 Antibody (B-6) (see ordering information below).
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GKLF Antibody (B-6) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 1-180 of human GKLF, also known as Kruppel-like factor 4 (KLF4). Anti-GKLF antibody (B-6) is validated for applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA), with species reactivity specific to human proteins. KLF4 is a transcription factor characterized by zinc finger domains, which enable DNA binding and regulate gene expression involved in cellular processes such as proliferation, differentiation, and stress response. KLF4 undergoes several post-translational modifications, including phosphorylation and acetylation, which influence stability, nuclear localization, and interaction with co-factors. These modifications fine-tune KLF4′s activity, enabling function as a transcriptional activator or repressor depending on cellular context. Predominantly expressed in epithelial tissues such as gastrointestinal tract and skin, KLF4 maintains epithelial homeostasis and regulates cell cycle arrest during growth and differentiation. Dysregulation of expression or post-translational modifications has been implicated in pathological conditions, including cancer, where KLF4 can function as either a tumor suppressor or oncogene. GKLF (B-6) monoclonal antibody serves as a valuable tool for studying KLF4 regulation and functional roles in development, cellular homeostasis, and disease processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.
Download thiomersal SDS
GKLF/KLF4 Antibody (B-6) References:
- Transactivation and growth suppression by the gut-enriched Krüppel-like factor (Krüppel-like factor 4) are dependent on acidic amino acid residues and protein-protein interaction. | Geiman, DE., et al. 2000. Nucleic Acids Res. 28: 1106-13. PMID: 10666450
- Krüppel-like factor 4 (gut-enriched Krüppel-like factor) inhibits cell proliferation by blocking G1/S progression of the cell cycle. | Chen, X., et al. 2001. J Biol Chem. 276: 30423-8. PMID: 11390382
- Opposing effects of Krüppel-like factor 4 (gut-enriched Krüppel-like factor) and Krüppel-like factor 5 (intestinal-enriched Krüppel-like factor) on the promoter of the Krüppel-like factor 4 gene. | Dang, DT., et al. 2002. Nucleic Acids Res. 30: 2736-41. PMID: 12087155
- Enterocyte differentiation marker intestinal alkaline phosphatase is a target gene of the gut-enriched Kruppel-like factor. | Hinnebusch, BF., et al. 2004. Am J Physiol Gastrointest Liver Physiol. 286: G23-30. PMID: 12919939
- Convergence of the thyroid hormone and gut-enriched Krüppel-like factor pathways in the context of enterocyte differentiation. | Siddique, A., et al. 2003. J Gastrointest Surg. 7: 1053-61; discussion 1061. PMID: 14675715
- Induction of p53 by GKLF is essential for inhibition of proliferation of vascular smooth muscle cells. | Wassmann, S., et al. 2007. J Mol Cell Cardiol. 43: 301-7. PMID: 17659301
- Krüppel-like factor 5 controls keratinocyte migration via the integrin-linked kinase. | Yang, Y., et al. 2008. J Biol Chem. 283: 18812-20. PMID: 18450752
- Krüppel-like factor 2 regulates trafficking and homeostasis of gammadelta T cells. | Odumade, OA., et al. 2010. J Immunol. 184: 6060-6. PMID: 20427763
- Krüppel-Like Factor 5 Promotes Epithelial Proliferation and DNA Damage Repair in the Intestine of Irradiated Mice. | Li, M., et al. 2015. Int J Biol Sci. 11: 1458-68. PMID: 26681925
- Krüppel-like Factor-4-Mediated Macrophage Polarization and Phenotypic Transitions Drive Intestinal Fibrosis in THP-1 Monocyte Models In Vitro. | Kanno, T., et al. 2024. Medicina (Kaunas). 60: PMID: 38792896
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GKLF/KLF4 Antibody (B-6) | sc-166229 | 200 µg/ml | $322.00 | |||
GKLF/KLF4 Antibody (B-6): m-IgG Fc BP-HRP Bundle | sc-540051 | 200 µg Ab; 10 µg BP | $361.00 | |||
GKLF/KLF4 Antibody (B-6): m-IgG1 BP-HRP Bundle | sc-541871 | 200 µg Ab; 20 µg BP | $361.00 | |||
GKLF/KLF4 Antibody (B-6) X | sc-166229 X | 200 µg/0.1 ml | $322.00 |