GK1 Antibody (F-5): sc-393555

GK1 Antibody (F-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects GK1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GK1 antibody (F-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GK1, or glycerol kinase 1, is a crucial enzyme that plays a significant role in glycerol metabolism, catalyzing the phosphorylation of glycerol to form glycerol phosphate, which is a vital intermediate in both glycolysis and gluconeogenesis. This enzymatic activity is essential for maintaining energy homeostasis and lipid metabolism, particularly in tissues such as the liver, kidney, and testis, where GK1 is highly expressed. GK1 consists of 559 amino acids and is classified as a mitochondrial peripheral membrane protein belonging to the FGGY kinase family. GK1 exists in three isoforms due to alternative splicing, which may contribute to functional diversity. Mutations in the genes encoding glycerol kinases can lead to glycerol kinase deficiency, a condition associated with hyperglycerolemia, psychomotor retardation, and osteoporosis, underscoring GK1′s importance in metabolic health.

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GK1 Antibody (F-5) References:

1. Glycerol as a correlate of impaired glucose tolerance: dissection of a complex system by use of a simple genetic trait.  |  Gaudet, D., et al. 2000. Am J Hum Genet. 66: 1558-68. PMID: 10736265
2. [Research progress on the glycerol kinase].  |  Guo, X., et al. 2002. Wei Sheng Wu Xue Bao. 42: 510-3. PMID: 12557562
3. Cloning of a human galactokinase gene (GK2) on chromosome 15 by complementation in yeast.  |  Lee, RT., et al. 1992. Proc Natl Acad Sci U S A. 89: 10887-91. PMID: 1438294
4. Disease genes and chromosomes: disease maps of the human genome. Chromosome 4.  |  Goldfrank, D., et al. 2003. Genet Test. 7: 351-72. PMID: 15000816
5. Generation and annotation of the DNA sequences of human chromosomes 2 and 4.  |  Hillier, LW., et al. 2005. Nature. 434: 724-31. PMID: 15815621
6. Lipolysis and the integrated physiology of lipid energy metabolism.  |  Wang, S., et al. 2008. Mol Genet Metab. 95: 117-26. PMID: 18762440
7. Complex glycerol kinase deficiency: molecular-genetic, cytogenetic, and clinical studies of five Japanese patients.  |  Matsumoto, T., et al. 1988. Am J Med Genet. 31: 603-16. PMID: 2852474
8. Comparison of the enzymatic activities of human galactokinase GALK1 and a related human galactokinase protein GK2.  |  Ai, Y., et al. 1995. Biochem Biophys Res Commun. 212: 687-91. PMID: 7542884
9. The glycerol kinase gene family: structure of the Xp gene, and related intronless retroposons.  |  Sargent, CA., et al. 1994. Hum Mol Genet. 3: 1317-24. PMID: 7987308
10. Mutations and phenotype in isolated glycerol kinase deficiency.  |  Walker, AP., et al. 1996. Am J Hum Genet. 58: 1205-11. PMID: 8651297

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GK1 Antibody (F-5) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-GK1 Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GK1 Antibody (F-5) Specifications

Alternate Product Name: GK1

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-132

Epitope Target: GK1

Epitope Species: human

Product Reactivity/Detection: GK1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for GK1 Antibody (F-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred GK1 antibody is GK1 Antibody (E-4): sc-398385.