GK1 Antibody (E-4): sc-398385
- GK1 Antibody (E-4) is a mouse monoclonal IgG2a κ GK1 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 13-31 near the N-terminus of GK1 of human origin
- GK1 Antibody (E-4) is recommended for detection of GK1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine and porcine
- Anti-GK1 Antibody (E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GK1 Antibody (E-4) for WB applications. These reagents are now offered in bundles with GK1 Antibody (E-4) (see ordering information below).
QUICK LINKS
GK1 Antibody (E-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects GK1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GK1 (E-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GK1, a 559 amino acid mitochondrial peripheral membrane protein, plays a crucial role in lipid metabolism as a key enzyme in the glycerol kinase family, catalyzing the phosphorylation of glycerol to form glycerol phosphate. This reaction is vital as glycerol phosphate serves as an important intermediate that can be converted into dihydroxyacetone phosphate (DHAP), a critical substrate for both glycolysis and gluconeogenesis. The regulation of glycerol uptake and metabolism by GK1 is essential for maintaining energy homeostasis, and mutations in the GK gene can lead to glycerol kinase deficiency, which is associated with serious health issues such as hyperglycerolemia, psychomotor retardation, and osteoporosis. GK1 exhibits high expression levels in the kidney, testis, and liver, and exists in three isoforms due to alternative splicing, highlighting functional diversity and importance in various physiological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GK1 Antibody (E-4) | sc-398385 | 200 µg/ml | $316.00 | |||
GK1 Antibody (E-4): m-IgG Fc BP-HRP Bundle | sc-530779 | 200 µg Ab; 10 µg BP | $354.00 | |||
GK1 Antibody (E-4): m-IgGκ BP-HRP Bundle | sc-524319 | 200 µg Ab, 40 µg BP | $354.00 | |||
GK1 Antibody (E-4): m-IgG2a BP-HRP Bundle | sc-547585 | 200 µg Ab; 10 µg BP | $354.00 | |||
GK1 Antibody (E-4) AC | sc-398385 AC | 500 µg/ml, 25% agarose | $416.00 | |||
GK1 Antibody (E-4) HRP | sc-398385 HRP | 200 µg/ml | $316.00 | |||
GK1 Antibody (E-4) FITC | sc-398385 FITC | 200 µg/ml | $330.00 | |||
GK1 Antibody (E-4) PE | sc-398385 PE | 200 µg/ml | $343.00 | |||
GK1 Antibody (E-4) Alexa Fluor® 488 | sc-398385 AF488 | 200 µg/ml | $357.00 | |||
GK1 Antibody (E-4) Alexa Fluor® 546 | sc-398385 AF546 | 200 µg/ml | $357.00 | |||
GK1 Antibody (E-4) Alexa Fluor® 594 | sc-398385 AF594 | 200 µg/ml | $357.00 | |||
GK1 Antibody (E-4) Alexa Fluor® 647 | sc-398385 AF647 | 200 µg/ml | $357.00 | |||
GK1 Antibody (E-4) Alexa Fluor® 680 | sc-398385 AF680 | 200 µg/ml | $357.00 | |||
GK1 Antibody (E-4) Alexa Fluor® 790 | sc-398385 AF790 | 200 µg/ml | $357.00 | |||
GK1 (E-4) Neutralizing Peptide | sc-398385 P | 100 µg/0.5 ml | $68.00 |