GIT1 Antibody (A-1): sc-365084

GIT1 Antibody (A-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects GIT1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-GIT1 antibody (A-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GIT1, known as GRK-interactor 1, plays a crucial role in the regulation of G protein-coupled receptor signaling by acting as a GTPase-activating protein for members of the ADP ribosylation factor family, which are essential for vesicular trafficking. GIT1 is primarily located in the cytoplasm and at the plasma membrane, where GIT1 interacts with various signaling molecules, thereby influencing cellular responses to external stimuli. Proper localization of GIT1 is vital for maintaining the balance of receptor signaling and ensuring effective cellular communication. Dysregulation of GIT1 can lead to altered receptor internalization and resensitization, impacting processes such as hormone signaling and neurotransmission. Using anti-GIT1 antibody (A-1) in research can provide valuable insights into the mechanisms of G protein-coupled receptor signaling and its implications in various physiological and pathological conditions.

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GIT1 Antibody (A-1) References:

1. The GIT family of ADP-ribosylation factor GTPase-activating proteins. Functional diversity of GIT2 through alternative splicing.  |  Premont, RT., et al. 2000. J Biol Chem. 275: 22373-80. PMID: 10896954
2. GIT1 is associated with ADHD in humans and ADHD-like behaviors in mice.  |  Won, H., et al. 2011. Nat Med. 17: 566-72. PMID: 21499268
3. Turning off the signal: desensitization of beta-adrenergic receptor function.  |  Hausdorff, WP., et al. 1990. FASEB J. 4: 2881-9. PMID: 2165947
4. G-protein-coupled receptor kinase interactor-1 (GIT1) is a new endothelial nitric-oxide synthase (eNOS) interactor with functional effects on vascular homeostasis.  |  Liu, S., et al. 2012. J Biol Chem. 287: 12309-20. PMID: 22294688
5. Phosphorylation of GIT1 tyrosine 321 is required for association with FAK at focal adhesions and for PDGF-activated migration of osteoblasts.  |  Ren, Y., et al. 2012. Mol Cell Biochem. 365: 109-18. PMID: 22302306
6. Identification of two tyrosine residues required for the intramolecular mechanism implicated in GIT1 activation.  |  Totaro, A., et al. 2014. PLoS One. 9: e93199. PMID: 24699139
7. Alpha-arrestins Aly1/Art6 and Aly2/Art3 regulate trafficking of the glycerophosphoinositol transporter Git1 and impact phospholipid homeostasis.  |  Robinson, BP., et al. 2022. Biol Cell. 114: 3-31. PMID: 34562280
8. Control of protein synthesis and memory by GluN3A-NMDA receptors through inhibition of GIT1/mTORC1 assembly.  |  Conde-Dusman, MJ., et al. 2021. Elife. 10: PMID: 34787081
9. An approach to the study of G-protein-coupled receptor kinases: an in vitro-purified membrane assay reveals differential receptor specificity and regulation by G beta gamma subunits.  |  Pei, G., et al. 1994. Proc Natl Acad Sci U S A. 91: 3633-6. PMID: 8170959
10. G protein-coupled receptors. III. New roles for receptor kinases and beta-arrestins in receptor signaling and desensitization.  |  Lefkowitz, RJ. 1998. J Biol Chem. 273: 18677-80. PMID: 9668034
11. G protein-coupled receptor kinases.  |  Pitcher, JA., et al. 1998. Annu Rev Biochem. 67: 653-92. PMID: 9759500
12. beta2-Adrenergic receptor regulation by GIT1, a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein.  |  Premont, RT., et al. 1998. Proc Natl Acad Sci U S A. 95: 14082-7. PMID: 9826657

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GIT1 Antibody (A-1) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-GIT1 Antibody (A-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GIT1 Antibody (A-1) Specifications

Alternate Product Name: GIT1

Product Clone Name: A-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 7-35

Epitope Target: GIT1

Epitope Species: rat

Product Reactivity/Detection: GIT1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for GIT1 Antibody (A-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin