girdin Antibody (H-6): sc-393757

Girdin Antibody (H-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects girdin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-girdin antibody (H-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Girdin, also known as CCDC88A (coiled-coil domain-containing protein 88A), APE, GRDN, GIV, or HkRP1, is a 1,871 amino acid protein that plays a pivotal role in cellular signaling and structural integrity. Girdin is primarily localized to the cell membrane and cytosol, where girdin interacts with phosphoinositides, enhancing the activity of phosphoinositide 3-kinase (PI3K) and promoting the phosphorylation of key proteins such as Akt1. This phosphorylation cascade is essential for regulating DNA replication and cellular proliferation, making girdin a critical player in cell growth and survival. Girdin is integral to cell migration, as girdin maintains the structural integrity of the actin cytoskeleton and regulates the formation of actin stress fibers. Girdin exists as a homodimer and can undergo phosphorylation, which leads to girdin delocalization from the cell membrane, thereby facilitating involvement in dynamic cell migration processes. Two isoforms of girdin arise from alternative splicing, contributing to functional diversity in various cellular contexts.

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girdin Antibody (H-6) References:

1. Identification and characterization of GIV, a novel Galpha i/s-interacting protein found on COPI, endoplasmic reticulum-Golgi transport vesicles.  |  Le-Niculescu, H., et al. 2005. J Biol Chem. 280: 22012-20. PMID: 15749703
2. A novel protein kinase B (PKB)/AKT-binding protein enhances PKB kinase activity and regulates DNA synthesis.  |  Anai, M., et al. 2005. J Biol Chem. 280: 18525-35. PMID: 15753085
3. A novel hook-related protein family and the characterization of hook-related protein 1.  |  Simpson, F., et al. 2005. Traffic. 6: 442-58. PMID: 15882442
4. Akt/PKB regulates actin organization and cell motility via Girdin/APE.  |  Enomoto, A., et al. 2005. Dev Cell. 9: 389-402. PMID: 16139227
5. Girdin, a novel actin-binding protein, and its family of proteins possess versatile functions in the Akt and Wnt signaling pathways.  |  Enomoto, A., et al. 2006. Ann N Y Acad Sci. 1086: 169-84. PMID: 17185515
6. Regulation of VEGF-mediated angiogenesis by the Akt/PKB substrate Girdin.  |  Kitamura, T., et al. 2008. Nat Cell Biol. 10: 329-37. PMID: 18264090
7. An actin-binding protein Girdin regulates the motility of breast cancer cells.  |  Jiang, P., et al. 2008. Cancer Res. 68: 1310-8. PMID: 18316593
8. A Novel Approach against Vascular Intimal Hyperplasia Through the Suppression of Girdin.  |  Miyachi, H., et al. 2015. Ann Vasc Dis. 8: 69-73. PMID: 26131024
9. Girdin Promotes Tumorigenesis and Chemoresistance in Lung Adenocarcinoma by Interacting with PKM2.  |  Cao, F., et al. 2022. Cancers (Basel). 14: PMID: 36428781
10. Girdin acts as an oncogene in gastric cancer by regulating AKT/GSK3β/β-catenin signaling.  |  Wang, Y., et al. 2023. Funct Integr Genomics. 23: 29. PMID: 36604355
11. Girdin regulates both migration and angiogenesis in pancreatic cancer cell lines.  |  Hayashi, Y., et al. 2023. Oncol Rep. 50: PMID: 37503752
12. Upregulation of girdin delays endothelial cell apoptosis via promoting engulfment of platelets.  |  Lan, Y., et al. 2023. Mol Biol Rep. 50: 8111-8120. PMID: 37548867

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-girdin Antibody (H-6) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-girdin Antibody (H-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

girdin Antibody (H-6) Specifications

Alternate Product Name: girdin

Product Clone Name: H-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1168-1294

Epitope Target: girdin

Epitope Species: human

Product Reactivity/Detection: girdin isoforms 1 and 2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for girdin Antibody (H-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin