Anti-GHS-R1 Antibody (E-7) recommended for detection of GHS-R1a and GHS-R1b of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-GHS-R1 Antibody (E-7): sc-374515

Anti-GHS-R1 Antibody (E-7) is rated 3.0 out of 5 by 2.
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Datasheets

    • Anti-GHS-R1 Antibody (E-7) is a mouse monoclonal IgG2a κ GHS-R1 antibody, cited in 8 publications, provided at 200 µg/ml
    • raised against amino acids 186-265 mapping within an internal region of GHS-R1 of human origin
    • Anti-GHS-R1 Antibody (E-7) is recommended for detection of GHS-R1a and GHS-R1b of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-GHS-R1 Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GHS-R1 (E-7): sc-374515.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for GHS-R1 Antibody (E-7) for WB applications. These reagents are now offered in bundles with GHS-R1 Antibody (E-7) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-374515: GHS-R1 (E-7) monoclonal antibody?

Asked by: Randy McDonald
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 1 out of 5 by from not detected any postive staining I bought this antibody two months ago,I detected with human Hela,rat brain and mouse brain,but not get a postive result
Date published: 2016-10-20
Rated 5 out of 5 by from Produced nice Western blot data of GHS Produced nice Western blot data of GHS-R1 expression in HeLa whole cell lysate. -SCBT QC
Date published: 2014-03-10
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