GHS-R1 Antibody (E-7): sc-374515

3.0(2)

- GHS-R1 Antibody (E-7) is a mouse monoclonal IgG_2a κ GHS-R1 antibody, cited in 10 publications, provided at 200 µg/ml
- raised against amino acids 186-265 mapping within an internal region of GHS-R1 of human origin
- GHS-R1 Antibody (E-7) is recommended for detection of GHS-R1a and GHS-R1b of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-GHS-R1 Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 or Alexa Fluor^® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor^® 680 or Alexa Fluor^® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR^®Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GHS-R1 (E-7): sc-374515.
- m-IgG Fc BP-HRP and m-IgG_2a BP-HRP are the preferred secondary detection reagents for GHS-R1 Antibody (E-7) for WB applications. These reagents are now offered in bundles with GHS-R1 Antibody (E-7) (see ordering information below).

References:

Expression of functional growth hormone secretagogue receptors in human pituitary adenomas: polymerase chain reaction, triple in-situ hybridization and cell culture studies. | Barlier, A., et al. 1999. J Neuroendocrinol. 11: 491-502. PMID: 10444306
Identification, characterization, and biological activity of specific receptors for natural (ghrelin) and synthetic growth hormone secretagogues and analogs in human breast carcinomas and cell lines. | Cassoni, P., et al. 2001. J Clin Endocrinol Metab. 86: 1738-45. PMID: 11297611
GH-releasing peptides and bone. | Cocchi, D., et al. 2005. J Endocrinol Invest. 28: 11-4. PMID: 16323823
Ghrelin stimulates insulin-induced glucose uptake in adipocytes. | Patel, AD., et al. 2006. Regul Pept. 134: 17-22. PMID: 16338009
Identification of a new G-protein-linked receptor for growth hormone secretagogues. | Pong, SS., et al. 1996. Mol Endocrinol. 10: 57-61. PMID: 8838145
Hypothalamic growth hormone secretagogue-receptor (GHS-R) expression is regulated by growth hormone in the rat. | Bennett, PA., et al. 1997. Endocrinology. 138: 4552-7. PMID: 9348177
Distribution of mRNA encoding the growth hormone secretagogue receptor in brain and peripheral tissues. | Guan, XM., et al. 1997. Brain Res Mol Brain Res. 48: 23-9. PMID: 9379845
Expression of the growth hormone secretagogue receptor in pituitary adenomas and other neuroendocrine tumors. | Korbonits, M., et al. 1998. J Clin Endocrinol Metab. 83: 3624-30. PMID: 9768675
Growth hormone-dependent regulation of pituitary GH secretagogue receptor (GHS-R) mRNA levels in the spontaneous dwarf Rat. | Kamegai, J., et al. 1998. Neuroendocrinology. 68: 312-8. PMID: 9822798

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession Number	Protein Accession Number	OMIM™ Number
Human	GHSR	Entrez Gene ID: 2693	3q26.31	NM_004122, NM_198407	UniProt ID: Q92847	604271
Mouse	Ghsr	Entrez Gene ID: 208188	3 A3	XM_017319513, NM_177330	UniProt ID: Q99P50	N/A

Full-length GHS-R1 protein sequence with immunogen highlighted:

                                        MWNATPSEEPGFNLTLADLDWDASPGNDSLGDELLQLFPAPLLAGVTATCVALFVVGIAGNLLTMLVVSRFRELRTTTNLYLSSMAFSDLLIFLCMPLDLVRLWQYRPWNFGDLLCKLFQFVSESCTYATVLTITALSVERYFAICFPLRAKVVVTKGRVKLVIFVIWAVAFCSAGPIFVLVGVEHENGTDPWDTNECRPTEFAVRSGLLTVMVWVSSIFFFLPVFCLTVLYSLIGRKLWRRRRGDAVVGASLRDQNHKQTVKMLAVVVFAFILCWLPFHVGRYLFSKSFEPGSLEIAQISQYCNLVSFVLFYLSAAINPILYNIMSKKYRVAVFRLLGFEPFSQRKLSTLKDESSRAWTESSINT

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GHS-R1 Antibody (E-7) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-GHS-R1 Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GHS-R1 Antibody (E-7) Specifications

Alternate Product Name

Ghrelin Receptor

Product Clone Name

E-7

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG_2a kappa light chain

Amino Acids

186-265

Epitope Target

GHS-R1

Epitope Species

human

Product Reactivity/Detection

GHS-R1a and GHS-R1b

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, ELISA

Vialing Information

Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

10 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

Mouse IgG_2a Binding Proteins

m-IgG_2a BP-HRP: sc-542731 (Mouse IgG_2a binding protein conjugated to horseradish peroxidase)
m-IgG_2a BP-B: sc-542734 (Mouse IgG_2a binding protein conjugated to biotin)
m-IgG_2a BP-FITC: sc-542733 (Mouse IgG_2a binding protein conjugated to fluorescein isothiocyanate)
m-IgG_2a BP-CFL 488: sc-542735 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 488)
m-IgG_2a BP-CFL 555: sc-542736 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 555)
m-IgG_2a BP-CFL 594: sc-542737 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 594)
m-IgG_2a BP-CFL 647: sc-542738 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 647)
m-IgG_2a BP-CFL 680: sc-542739 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 680)
m-IgG_2a BP-CFL 790: sc-542740 (Mouse IgG_2a binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

AC: Agarose AF488: Alexa Fluor^® 488 AF546: Alexa Fluor^® 546 AF594: Alexa Fluor^® 594 AF647: Alexa Fluor^® 647 AF680: Alexa Fluor^® 680 AF790: Alexa Fluor^® 790 FITC: Fluoroscein HRP: Horseradish Peroxidase PE: Phycoerythrin

Primary antibodies for non-mammalian targets are useful in detecting proteins of plant, viral, bacterial, avian, fish, yeast and invertebrates. Some examples include Drosophila, zebrafish, Xenopus, Arabidopsis, C. elegans, Clostridium, Campylobacter, Heliobacter pylori, Hepatitis, HIV, HPV, HSV, Influenza, Klebsiella, M. tuberculosis, Plasmodium, Pseudomonas, RSV, Saccharomyces cerevisiae, Salmonella, Schistosoma, Shigella and Staphylococcus.

Santa Cruz Biotechnology monoclonal antibodies can be used in assays including flow cytometry, immunoprecipitation, Western blot analysis, and immunostaining. Anti-GHS-R1 Antibody (E-7) is recommended for use in the following applications: WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), ELISA (Enzyme-Linked Immunosorbent Assay).

Mouse secondary antibodies, control sera and control immunoglobulins are also offered to be used in combination with our primary monoclonal antibodies.

Tissue extracts, nuclear extracts and whole cell lysates are used with Santa Cruz Biotechnology monoclonal antibodies for western blotting or immunoprecipitation positive controls. Nuclear extracts are ideal for western blot analysis or gel shift assays positive controls. Phospho-enriched whole cell lysates and tissue extracts are available for phospho-specific antibody positive controls.

Monoclonal secondary antibodies are offered in various conjugated forms, including: Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), and TRITC (tetramethyl rhodamine isothiocyanate) (R), for research applications like Flow Cytometry (FCM), Immunofluorescence (IF), Immunohistochemistry (IHC) staining and Western Blotting (WB). Not intended for diagnostic or therapeutic use.

Affinity purified immunoglobulins (IgGs)—conjugated and unconjugated—are available as negative controls for flow cytometry (FCM), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP) and Western Blotting (WB) applications.

Normal sera for immunohistochemistry (IHC) applications are available as neat serum for blocking reagents. The serum which is used should match the species of the secondary antibody.

What is the recommended starting dilution for Western blot for sc-374515: GHS-R1 (E-7) monoclonal antibody?

Asked by: Randy McDonald

The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting

Answered by: Technical Support

Date published: 2017-03-22

Rated 1 out of 5 by maoying from not detected any postive staining I bought this antibody two months ago，I detected with human Hela，rat brain and mouse brain，but not get a postive result

Date published: 2016-10-20

Rated 5 out of 5 by MS from Produced nice Western blot data of GHS Produced nice Western blot data of GHS-R1 expression in HeLa whole cell lysate. -SCBT QC

Date published: 2014-03-10

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