GFP Antibody (C-2): sc-390394

GFP Antibody (C-2) is a mouse monoclonal IgG2b kappa light chain antibody specific for an epitope mapping between amino acids 167-182 within an internal region of green fluorescent protein (GFP) of Aequorea victoria origin. GFP is a 27 kDa protein that has revolutionized molecular and cellular biology by enabling the monitoring of gene expression and protein localization in vivo without the need for exogenous substrates or cofactors, thanks to its intrinsic fluorescence arising from a unique chromophore formed within its structure. Anti-GFP antibody (C-2) is suitable for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), and flow cytometry (FCM). While GFP itself does not naturally interact with a wide range of proteins, GFP serves extensively as a fusion tag in expression vectors to study specific proteins′ interactions and localization within the cell. By fusing GFP to proteins of interest, researchers can visualize and track proteins like transcription factors, signaling molecules, and structural proteins, thereby gaining insights into processes such as protein-protein interactions, intracellular trafficking, and cellular dynamics. Enhanced variants like EGFP have been developed for increased fluorescence intensity and stability, further improving their utility in advanced imaging techniques. GFP (C-2) antibody facilitates the detection and analysis of GFP and GFP-tagged proteins across various biological systems, making GFP (C-2) monoclonal antibody an invaluable tool in biological research.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GFP Antibody (C-2) has 40 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GFP Antibody (C-2) Specifications

Alternate Product Name: GFP

Product Clone Name: C-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 167-182

Epitope Target: GFP

Epitope Species: Aequorea victoria

Product Reactivity/Detection: GFP and GFP mutant fusion proteins

Species Reactivity/Detection: N/A

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 40 citations in various scientific publications

Available Conjugates/Forms for GFP Antibody (C-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred GFP antibody is GFP Antibody (B-2): sc-9996.