Gemin8 Antibody (A-12): sc-376419

Gemin8 Antibody (A-12) is a mouse monoclonal IgG1 antibody that detects Gemin8 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Gemin8 is a crucial 242 amino acid protein encoded by the mouse gene Gemin8, and plays a significant role as a key component of the survival of motor neurons (SMN) complex, which is essential for the assembly of small nuclear ribonucleoproteins (snRNPs) that are vital for pre-mRNA splicing. The SMN complex, which includes Gemin8 along with Gemins 2-7 and unrip, acts as an assembly machine for snRNPs, and proper function is critical for maintaining neuronal health. Gemin8 directly binds to the SMN protein, facilitating interaction with the Gemin6/Gemin7 heterodimer, which is important for organizing Sm proteins necessary for the formation of Sm rings on snRNA targets. Disruption of Gemin8 function can lead to impaired snRNP assembly, highlighting importance in the pathogenesis of spinal muscular atrophy, a neurodegenerative disease characterized by the loss of motor neurons. Anti-Gemin8 antibody (A-12) is an invaluable tool for researchers studying the molecular mechanisms underlying this disease and the broader implications of the SMN complex in cellular function.

The preferred Gemin8 antibody is Gemin8 Antibody (1F8): sc-130669.