Gemin8 Antibody (1F8): sc-130669
- Gemin8 Antibody (1F8) is a mouse monoclonal IgG2a κ Gemin8 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against recombinant Gemin8 of human origin
- Gemin8 Antibody (1F8) is recommended for detection of Gemin8 of mouse, rat, human and canine origin by WB, IP, IF and ELISA
- Anti-Gemin8 Antibody (1F8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Gemin8 Antibody (1F8) for WB applications. These reagents are now offered in bundles with Gemin8 Antibody (1F8) (see ordering information below).
Gemin8 (1F8) is a mouse monoclonal IgG2a kappa light chain antibody that detects Gemin8 protein of mouse, rat, human and canine origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA). Anti-Gemin8 antibody (1F8) is available as non-conjugated form, as well as multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. Gemin8 is a 242 amino acid protein encoded by the mouse gene Gemin8. Gemin8, along with Gemins 2-7 and unrip, is a major component of the large multiprotein survival of motor neurons (SMN) complex. The survival of motor neurons protein, a product of the disease gene of the common neurodegenerative disease spinal muscular atrophy, is also part of the SMN complex. The SMN complex is a modular composition of proteins with SMN, Gemin8, and Gemin7 in its center. The SMN complex functions as an assembly machine for small nuclear ribonucleoproteins (snRNPs)-the major components of the spliceosome. Gemin8 binds directly to SMN and mediates interaction with the Gemin6/Gemin7 heterodimer. Loss of Gemin6, Gemin7, and unrip interaction with SMN due to Gemin8 knockdown affects snRNP assembly by impairing SMN complex association with Sm proteins but not with snRNAs. The Gemin6/Gemin7 complex binds to Sm proteins and might help organize Sm proteins for formation of Sm rings on snRNA targets.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Gemin8 Antibody (1F8) References:
- The SMN complex is associated with snRNPs throughout their cytoplasmic assembly pathway. | Massenet, S., et al. 2002. Mol Cell Biol. 22: 6533-41. PMID: 12192051
- Gemin proteins are required for efficient assembly of Sm-class ribonucleoproteins. | Shpargel, KB. and Matera, AG. 2005. Proc Natl Acad Sci U S A. 102: 17372-7. PMID: 16301532
- Gemin8 is a novel component of the survival motor neuron complex and functions in small nuclear ribonucleoprotein assembly. | Carissimi, C., et al. 2006. J Biol Chem. 281: 8126-34. PMID: 16434402
- Multiprotein complexes of the survival of motor neuron protein SMN with Gemins traffic to neuronal processes and growth cones of motor neurons. | Zhang, H., et al. 2006. J Neurosci. 26: 8622-32. PMID: 16914688
- Gemin8 is required for the architecture and function of the survival motor neuron complex. | Carissimi, C., et al. 2006. J Biol Chem. 281: 37009-16. PMID: 17023415
- A comprehensive interaction map of the human survival of motor neuron (SMN) complex. | Otter, S., et al. 2007. J Biol Chem. 282: 5825-33. PMID: 17178713
- Synergistic inhibition of pancreatic adenocarcinoma cell growth by trichostatin A and gemcitabine. | Donadelli, M., et al. 2007. Biochim Biophys Acta. 1773: 1095-106. PMID: 17555830
- Ribonucleoprotein assembly defects correlate with spinal muscular atrophy severity and preferentially affect a subset of spliceosomal snRNPs. | Gabanella, F., et al. 2007. PLoS One. 2: e921. PMID: 17895963
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gemin8 Antibody (1F8) | sc-130669 | 200 µg/ml | $322.00 | |||
Gemin8 Antibody (1F8): m-IgG Fc BP-HRP Bundle | sc-525741 | 200 µg Ab; 10 µg BP | $361.00 | |||
Gemin8 Antibody (1F8): m-IgG2a BP-HRP Bundle | sc-546391 | 200 µg Ab; 10 µg BP | $361.00 | |||
Gemin8 Antibody (1F8) AC | sc-130669 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Gemin8 Antibody (1F8) HRP | sc-130669 HRP | 200 µg/ml | $322.00 | |||
Gemin8 Antibody (1F8) FITC | sc-130669 FITC | 200 µg/ml | $336.00 | |||
Gemin8 Antibody (1F8) PE | sc-130669 PE | 200 µg/ml | $349.00 | |||
Gemin8 Antibody (1F8) Alexa Fluor® 488 | sc-130669 AF488 | 200 µg/ml | $364.00 | |||
Gemin8 Antibody (1F8) Alexa Fluor® 546 | sc-130669 AF546 | 200 µg/ml | $364.00 | |||
Gemin8 Antibody (1F8) Alexa Fluor® 594 | sc-130669 AF594 | 200 µg/ml | $364.00 | |||
Gemin8 Antibody (1F8) Alexa Fluor® 647 | sc-130669 AF647 | 200 µg/ml | $364.00 | |||
Gemin8 Antibody (1F8) Alexa Fluor® 680 | sc-130669 AF680 | 200 µg/ml | $364.00 | |||
Gemin8 Antibody (1F8) Alexa Fluor® 790 | sc-130669 AF790 | 200 µg/ml | $364.00 |