Gem Antibody (G-1): sc-166891
- Gem Antibody (G-1) is a mouse monoclonal IgG2a κ Gem antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1-85 mapping at the N-terminus of Gem of human origin
- Gem Antibody (G-1) is recommended for detection of Gem of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Gem Antibody (G-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Gem Antibody (G-1) for WB applications. These reagents are now offered in bundles with Gem Antibody (G-1) (see ordering information below).
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SEE ALSO...
Gem Antibody (G-1) is a mouse monoclonal IgG2a kappa light chain antibody that detects Gem protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Gem antibody (G-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Gem protein, a member of the Rad/Gem/Kir (RGK) subfamily of Ras-related GTPases, plays a crucial role in regulating cellular morphology and cytoskeletal dynamics. Gem protein is located in the cytoplasm and interacts with microtubules and microfilaments, which is essential for maintaining cell shape and facilitating cellular processes such as neurite extension and flattening in neuroblastoma cells. This cytoplasmic localization allows Gem protein to influence the organization of the cytoskeleton, thereby impacting neuronal differentiation and development. Gem protein′s interaction with beta-subunits is vital for regulating calcium channel expression at the cell surface, highlighting its importance in calcium signaling pathways. Human Gem gene is mapped to chromosome 8q22.1, underscoring its relevance in both normal physiological processes and potential pathological conditions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Gem Antibody (G-1) References:
- Regulation of Ca2+ channel expression at the cell surface by the small G-protein kir/Gem. | Béguin, P., et al. 2001. Nature. 411: 701-6. PMID: 11395774
- The Gem GTP-binding protein promotes morphological differentiation in neuroblastoma. | Leone, A., et al. 2001. Oncogene. 20: 3217-25. PMID: 11423971
- The Ras-like GTPase Gem is involved in cell shape remodelling and interacts with the novel kinesin-like protein KIF9. | Piddini, E., et al. 2001. EMBO J. 20: 4076-87. PMID: 11483511
- Effects of phosphorylation on function of the Rad GTPase. | Moyers, JS., et al. 1998. Biochem J. 333 (Pt 3): 609-14. PMID: 9677319
- The ras-related protein rad associates with the cytoskeleton in a non-lipid-dependent manner. | Bilan, PJ., et al. 1998. Exp Cell Res. 242: 391-400. PMID: 9683526
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gem Antibody (G-1) | sc-166891 | 200 µg/ml | $322.00 | |||
Gem Antibody (G-1): m-IgG Fc BP-HRP Bundle | sc-525772 | 200 µg Ab; 10 µg BP | $361.00 | |||
Gem Antibody (G-1): m-IgG2a BP-HRP Bundle | sc-546411 | 200 µg Ab; 10 µg BP | $361.00 | |||
Gem Antibody (G-1) AC | sc-166891 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Gem Antibody (G-1) HRP | sc-166891 HRP | 200 µg/ml | $322.00 | |||
Gem Antibody (G-1) FITC | sc-166891 FITC | 200 µg/ml | $336.00 | |||
Gem Antibody (G-1) PE | sc-166891 PE | 200 µg/ml | $349.00 | |||
Gem Antibody (G-1) Alexa Fluor® 488 | sc-166891 AF488 | 200 µg/ml | $364.00 | |||
Gem Antibody (G-1) Alexa Fluor® 546 | sc-166891 AF546 | 200 µg/ml | $364.00 | |||
Gem Antibody (G-1) Alexa Fluor® 594 | sc-166891 AF594 | 200 µg/ml | $364.00 | |||
Gem Antibody (G-1) Alexa Fluor® 647 | sc-166891 AF647 | 200 µg/ml | $364.00 | |||
Gem Antibody (G-1) Alexa Fluor® 680 | sc-166891 AF680 | 200 µg/ml | $364.00 | |||
Gem Antibody (G-1) Alexa Fluor® 790 | sc-166891 AF790 | 200 µg/ml | $364.00 |