Anti-GDF-9 Antibody (C-6) recommended for detection of GDF-9 of human origin by WB, IP, IF and ELISA

Anti-GDF-9 Antibody (C-6): sc-514933

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Datasheets

    • Anti-GDF-9 Antibody (C-6) is a mouse monoclonal IgG2a (kappa light chain) GDF-9 antibody provided at 200 µg/ml
    • raised against amino acids 331-454 mapping at the C-terminus of GDF-9 of human origin
    • GDF-9 Antibody (C-6) is recommended for detection of GDF-9 of human origin by WB, IP, IF and ELISA
    • Anti-GDF-9 Antibody (C-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GDF-9 (C-6): sc-514933.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for GDF-9 Antibody (C-6) for WB applications. These reagents are now offered in bundles with GDF-9 Antibody (C-6) (see ordering information below).

What blocking solution should I use with GDF-9 (C-6): sc-514933 monoclonal antibody for Western blot?

Asked by: AbPolly
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-28
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