GD3 Synthase Antibody (B-11): sc-390123

GD3 Synthase Antibody (B-11) is a mouse monoclonal IgG1 antibody that detects GD3 Synthase protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GD3 Synthase (B-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GD3 Synthase, also known as GD3S, SIAT8, ST8SiaI, or ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1, is a type II membrane protein located in the Golgi apparatus, where GD3 Synthase plays a crucial role in the biosynthesis of gangliosides by catalyzing the transfer of sialic acid from CMP-sialic acid to GM3, resulting in the production of GD3 and GT3 gangliosides. These gangliosides are essential for various cellular processes, including cell adhesion and growth, particularly in malignant cells, highlighting their importance in cancer biology. GD3 Synthase is a member of glycosyltransferase family 29 and has been shown to down-regulate matrix metalloproteinase-9 promoter activity in response to tumor necrosis factor-alpha by interacting with NF-kappaB and activation protein-1 sites in the MMP-9 promoter. Additionally, GD3 Synthase has an apoptotic effect on ECV304 cells through the downregulation of Bcl-2 expression via the dephosphorylation of AKT and CREB, further underscoring its significance in cellular signaling pathways.

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GD3 Synthase Antibody (B-11) References:

1. Down-regulation of GD3 ganglioside and its O-acetylated derivative by stable transfection with antisense vector against GD3-synthase gene expression in hamster melanoma cells: effects on cellular growth, melanogenesis, and dendricity.  |  Birklé, S., et al. 2000. J Neurochem. 74: 547-54. PMID: 10646505
2. GD3 synthase gene expression in PC12 cells results in the continuous activation of TrkA and ERK1/2 and enhanced proliferation.  |  Fukumoto, S., et al. 2000. J Biol Chem. 275: 5832-8. PMID: 10681573
3. Genes modulated by expression of GD3 synthase in Chinese hamster ovary cells. Evidence that the Tis21 gene is involved in the induction of GD3 9-O-acetylation.  |  Satake, H., et al. 2003. J Biol Chem. 278: 7942-8. PMID: 12493756
4. Isolation and functional analysis of the melanoma specific promoter region of human GD3 synthase gene.  |  Furukawa, K., et al. 2003. Biochim Biophys Acta. 1627: 71-8. PMID: 12818424
5. Disialoganglioside (GD3) synthase gene expression suppresses vascular smooth muscle cell responses via the inhibition of ERK1/2 phosphorylation, cell cycle progression, and matrix metalloproteinase-9 expression.  |  Moon, SK., et al. 2004. J Biol Chem. 279: 33063-70. PMID: 15175338
6. Calnexin suppresses GD3 synthase-induced apoptosis.  |  Tomassini, B., et al. 2004. FASEB J. 18: 1553-5. PMID: 15319364
7. GD3 synthase regulates epithelial-mesenchymal transition and metastasis in breast cancer.  |  Sarkar, TR., et al. 2015. Oncogene. 34: 2958-67. PMID: 25109336
8. GD3 synthase deletion alters retinal structure and impairs visual function in mice.  |  Abreu, CA., et al. 2021. J Neurochem. 158: 694-709. PMID: 34081777
9. Role of GD3 Synthase ST8Sia I in Cancers.  |  Kasprowicz, A., et al. 2022. Cancers (Basel). 14: PMID: 35267607
10. The biological role and immunotherapy of gangliosides and GD3 synthase in cancers.  |  Cao, S., et al. 2023. Front Cell Dev Biol. 11: 1076862. PMID: 36824365
11. Influence of N-glycosylation and N-glycan trimming on the activity and intracellular traffic of GD3 synthase.  |  Martina, JA., et al. 1998. J Biol Chem. 273: 3725-31. PMID: 9452504
12. Embryonic stem cells with a disrupted GD3 synthase gene undergo neuronal differentiation in the absence of b-series gangliosides.  |  Kawai, H., et al. 1998. J Biol Chem. 273: 19634-8. PMID: 9677390

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GD3 Synthase Antibody (B-11) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-GD3 Synthase Antibody (B-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GD3 Synthase Antibody (B-11) Specifications

Alternate Product Name: GD3 Synthase

Product Clone Name: B-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ lambda light chain

Amino Acids: 65-140

Epitope Target: GD3 Synthase

Epitope Species: human

Product Reactivity/Detection: GD3 Synthase

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ lambda light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for GD3 Synthase Antibody (B-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin