GCNF Antibody (B-2): sc-271733
- GCNF Antibody (B-2) is a mouse monoclonal IgG2a κ GCNF antibody provided at 200 µg/ml
- raised against amino acids 181-480 mapping at the C-terminus of GCNF of human origin
- GCNF Antibody (B-2) is recommended for detection of GCNF of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-GCNF Antibody (B-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-271733 X, 200 µg/0.1 ml)
- m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GCNF Antibody (B-2) for WB applications. These reagents are now offered in bundles with GCNF Antibody (B-2) (see ordering information below).
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SEE ALSO...
GCNF Antibody (B-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects GCNF protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GCNF (B-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Germ cell nuclear factor (GCNF), also known as NR6A1, plays a crucial role in regulating neurogenesis and germ cell development, making GCNF essential for proper reproductive function. GCNF is primarily located in the nucleus, where GCNF can homodimerize and bind to specific DNA sequences, thereby influencing gene expression. This nuclear localization allows GCNF to regulate paracrine interactions between oocytes and somatic cells, particularly through modulation of bone morphogenetic protein-15 and growth differentiation factor-9, which are critical for female fertility. GCNF is predominantly expressed in testis, specifically in spermatocytes and round spermatids, highlighting GCNF′s importance in male reproductive biology. Notably, GCNF gene produces three alternatively spliced transcript variants, which may contribute to diverse regulatory functions in germ cell development.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GCNF Antibody (B-2) References:
- Characterization and DNA-binding properties of GRF, a novel monomeric binding orphan receptor related to GCNF and betaFTZ-F1. | Charles, JP., et al. 1999. Eur J Biochem. 266: 181-90. PMID: 10542063
- Oocytes and embryos of Xenopus laevis express two different isoforms of germ cell nuclear factor (GCNF, NR6A1). | Schohl, A., et al. 2002. Mech Dev. 118: 261-4. PMID: 12351198
- GCNF-dependent repression of BMP-15 and GDF-9 mediates gamete regulation of female fertility. | Lan, ZJ., et al. 2003. EMBO J. 22: 4070-81. PMID: 12912906
- The expression level of the orphan nuclear receptor GCNF (germ cell nuclear factor) is critical for neuronal differentiation. | Sattler, U., et al. 2004. Mol Endocrinol. 18: 2714-26. PMID: 15297607
- A boundary for histone acetylation allows distinct expression patterns of the Ad4BP/SF-1 and GCNF loci in adrenal cortex cells. | Ishihara, SL. and Morohashi, K. 2005. Biochem Biophys Res Commun. 329: 554-62. PMID: 15737622
- Cloning of a novel orphan receptor (GCNF) expressed during germ cell development. | Chen, F., et al. 1994. Mol Endocrinol. 8: 1434-44. PMID: 7854358
- cDNA cloning of two closely related forms of human germ cell nuclear factor (GCNF). | Kapelle, M., et al. 1997. Biochim Biophys Acta. 1352: 13-7. PMID: 9177477
- Cloning, expression analysis and chromosomal localization of the human nuclear receptor gene GCNF. | Agoulnik, IY., et al. 1998. FEBS Lett. 424: 73-8. PMID: 9537518
- The murine nuclear orphan receptor GCNF is expressed in the XY body of primary spermatocytes. | Bauer, UM., et al. 1998. FEBS Lett. 439: 208-14. PMID: 9845324
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GCNF Antibody (B-2) | sc-271733 | 200 µg/ml | $322.00 | |||
GCNF Antibody (B-2): m-IgGκ BP-HRP Bundle | sc-522042 | 200 µg Ab, 40 µg BP | $361.00 | |||
GCNF Antibody (B-2): m-IgG2a BP-HRP Bundle | sc-546105 | 200 µg Ab; 10 µg BP | $361.00 | |||
GCNF Antibody (B-2) X | sc-271733 X | 200 µg/0.1 ml | $322.00 | |||
GCNF Antibody (B-2) AC | sc-271733 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GCNF Antibody (B-2) HRP | sc-271733 HRP | 200 µg/ml | $322.00 | |||
GCNF Antibody (B-2) FITC | sc-271733 FITC | 200 µg/ml | $336.00 | |||
GCNF Antibody (B-2) PE | sc-271733 PE | 200 µg/ml | $349.00 | |||
GCNF Antibody (B-2) Alexa Fluor® 488 | sc-271733 AF488 | 200 µg/ml | $364.00 | |||
GCNF Antibody (B-2) Alexa Fluor® 546 | sc-271733 AF546 | 200 µg/ml | $364.00 | |||
GCNF Antibody (B-2) Alexa Fluor® 594 | sc-271733 AF594 | 200 µg/ml | $364.00 | |||
GCNF Antibody (B-2) Alexa Fluor® 647 | sc-271733 AF647 | 200 µg/ml | $364.00 | |||
GCNF Antibody (B-2) Alexa Fluor® 680 | sc-271733 AF680 | 200 µg/ml | $364.00 | |||
GCNF Antibody (B-2) Alexa Fluor® 790 | sc-271733 AF790 | 200 µg/ml | $364.00 |