GCAP2 Antibody (G-10): sc-166056

GCAP2 Antibody (G-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects GCAP2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). GCAP2 (G-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GCAP2 plays a crucial role in the retina, specifically in the regulation of phototransduction, which is essential for converting light signals into visual information. GCAP2 is primarily located in the outer segments of rod photoreceptors, where GCAP2 interacts with guanylate cyclase to modulate cGMP levels in response to changes in intracellular calcium concentrations. This regulation is vital for the recovery of photoreceptors after exposure to light, ensuring that vision can adapt to varying light conditions. GCAP2 is a product of gene duplication and is closely related to GCAP1, with both proteins sharing over 90% similarity in their amino acid sequences and three conserved calcium-binding sites. GCAP1 and GCAP2 genes are located on human chromosome 6p21.1, highlighting their evolutionary significance and functional importance in visual signaling pathways.

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GCAP2 Antibody (G-10) References:

1. Conformational changes in guanylyl cyclase-activating protein 1 (GCAP1) and its tryptophan mutants as a function of calcium concentration.  |  Sokal, I., et al. 1999. J Biol Chem. 274: 19829-37. PMID: 10391927
2. Guanylyl cyclase activating protein. A calcium-sensitive regulator of phototransduction.  |  Gorczyca, WA., et al. 1995. J Biol Chem. 270: 22029-36. PMID: 7665624
3. Molecular characterization of human and mouse photoreceptor guanylate cyclase-activating protein (GCAP) and chromosomal localization of the human gene.  |  Subbaraya, I., et al. 1994. J Biol Chem. 269: 31080-9. PMID: 7983048
4. Localization of guanylate cyclase-activating protein 2 in mammalian retinas.  |  Otto-Bruc, A., et al. 1997. Proc Natl Acad Sci U S A. 94: 4727-32. PMID: 9114059
5. The human GCAP1 and GCAP2 genes are arranged in a tail-to-tail array on the short arm of chromosome 6 (p21.1).  |  Surguchov, A., et al. 1997. Genomics. 39: 312-22. PMID: 9119368
6. Changes in biological activity and folding of guanylate cyclase-activating protein 1 as a function of calcium.  |  Rudnicka-Nawrot, M., et al. 1998. Biochemistry. 37: 248-57. PMID: 9425045

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GCAP2 Antibody (G-10) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-GCAP2 Antibody (G-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GCAP2 Antibody (G-10) Specifications

Alternate Product Name: GCAP2

Product Clone Name: G-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 130-201

Epitope Target: GCAP2

Epitope Species: mouse

Product Reactivity/Detection: GCAP2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for GCAP2 Antibody (G-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin