Date published: 2026-6-11
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G<sub>&beta;</sub><sub> Antibody (</sub>F-8) - Western Blotting - Image 354707
Gβ Antibody (F-8): sc-136975. Western blot analysis of Gβ expression in Jurkat (A), U-251-MG (B), NIH/3T3 (C), AMJ2-C8 (D), C6 (E) and NRK (F) whole cell lysates.
Gβ Antibody (F-8): sc-136975. Western blot analysis of Gβ expression in Jurkat (A), U-251-MG (B), NIH/3T3 (C), AMJ2-C8 (D), C6 (E) and NRK (F) whole cell lysates.

Gβ Antibody (F-8): sc-136975

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Datasheets
  • Gβ Antibody (F-8) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
  • raised against amino acids 1-300 mapping at the N-terminus of Gβ 2 of human origin
  • recommended for detection of Gβ 1, Gβ 2, Gβ 3 and Gβ 4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Gβ Antibody (F-8) for WB and IHC(P) applications. These reagents are now offered in bundles with Gβ Antibody (F-8) (see ordering information below).
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Additional Heterotrimeric G Protein Antibodies including G α 11, G α 12, G α 13, G α i1, G α i2, G α i3, G α o, G α olf, G α q, G α q11, G α q14, G α q8, G α t, G α t1, G β and G β 1

Gβ Antibody (F-8) is a mouse monoclonal IgG1 antibody that detects Gβ in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Gβ, a crucial component of heterotrimeric G proteins, plays a significant role in transmitting signals from cell surface receptors to intracellular effectors, thereby influencing numerous physiological processes. Gβ subunits are essential regulators of G protein alpha subunits and certain signal transduction receptors, which are vital for maintaining cellular communication and response to external stimuli. Notably, Gβ5, one of the five members of the β-subunit family, exhibits unique characteristics, being only 50% homologous to the other β subunits, Gβ1-4, which share at least 83% homology. This distinctiveness is important as Gβ5 is predominantly expressed in the brain, pancreas, kidney, and heart, suggesting specialized functions in these organs. anti-Gβ antibody (F-8) is an invaluable tool for researchers exploring the intricate signaling pathways mediated by G proteins and their implications in health and disease.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Gβ Antibody (F-8) References:

  1. G beta association and effector interaction selectivities of the divergent G gamma subunit G gamma(13).  |  Blake, BL., et al. 2001. J Biol Chem. 276: 49267-74. PMID: 11675383
  2. Differential requirement of G alpha12, G alpha13, G alphaq, and G beta gamma for endothelin-1-induced c-Jun NH2-terminal kinase and extracellular signal-regulated kinase activation.  |  Arai, K., et al. 2003. Mol Pharmacol. 63: 478-88. PMID: 12606754
  3. Effects of G-protein-specific antibodies and G beta gamma subunits on the muscarinic receptor-operated cation current in guinea-pig ileal smooth muscle cells.  |  Yan, HD., et al. 2003. Br J Pharmacol. 139: 605-15. PMID: 12788820
  4. The Emerging Role of Gβ Subunits in Human Genetic Diseases.  |  Malerba, N., et al. 2019. Cells. 8: PMID: 31817184
  5. Encephalopathy-causing mutations in Gβ1 (GNB1) alter regulation of neuronal GIRK channels.  |  Reddy, HP., et al. 2021. iScience. 24: 103018. PMID: 34522861
  6. G-protein couples MAPK cascade through maize heterotrimeric Gβ subunit.  |  Chen, Y., et al. 2022. Plant Cell Rep. 41: 1763-1774. PMID: 35737098
  7. Abscisic acid-controlled redox proteome of Arabidopsis and its regulation by heterotrimeric Gβ protein.  |  Smythers, AL., et al. 2022. New Phytol. 236: 447-463. PMID: 35766993
  8. Lumipulse G β-Amyloid Ratio Test for the Diagnosis of Alzheimer Disease.  |  Lau, K. and Lin, KW. 2023. Am Fam Physician. 107: 550-551. PMID: 37192086
  9. G beta gamma binds directly to the G protein-gated K+ channel, IKACh.  |  Krapivinsky, G., et al. 1995. J Biol Chem. 270: 29059-62. PMID: 7493925
  10. Structural elements of G alpha subunits that interact with G beta gamma, receptors, and effectors.  |  Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Gβ Antibody (F-8)

sc-136975
200 µg/ml
$322.00

Gβ Antibody (F-8): m-IgG Fc BP-HRP Bundle

sc-537383
200 µg Ab; 10 µg BP
$361.00

Gβ Antibody (F-8): m-IgGκ BP-HRP Bundle

sc-534634
200 µg Ab; 40 µg BP
$361.00

Gβ Antibody (F-8): m-IgG1 BP-HRP Bundle

sc-545168
200 µg Ab; 20 µg BP
$361.00

Can G&beta; (F-8): sc-136975 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: AbPolly
Thank you for your question. Yes, G&beta; (F-8): sc-136975 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from Strong cytoplasmic staining in IHCStrong cytoplasmic staining in IHC in human transfected HEK293 cells.
Date published: 2016-11-29
Rated 5 out of 5 by from Good specificity in WB in mouse brain tissueGood specificity in WB in mouse brain tissue extract. -SCBT QC
Date published: 2015-02-08
Rated 4 out of 5 by from Strong cytoplasmic and membrane stainingStrong cytoplasmic and membrane staining in formalin fixed human urinary bladder tissue in IHC. -SCBT QC
Date published: 2013-06-09
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G β Antibody (F-8) is rated 4.3 out of 5 by 3.
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