G_β Antibody (H-1): sc-166123

Gβ Antibody (H-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Gβ protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Gβ (H-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Gβ protein plays a crucial role in cellular signaling as part of heterotrimeric G proteins, which are essential for transmitting signals from cell surface receptors to intracellular effectors. These receptors are responsible for detecting a wide array of extracellular stimuli, such as hormones and neurotransmitters, while Gβ subunits help regulate the activity of G protein alpha subunits and certain signal transduction pathways. Notably, Gβ subunit family consists of five members in mammals, with Gβ1-4 sharing at least 83% homology, whereas Gβ5 exhibits only 50% homology to the others. Gβ5 is particularly significant as Gβ5 is highly expressed in the brain, pancreas, kidney, and heart, indicating potential involvement in various physiological processes. Gβ proteins′ ability to modulate signaling pathways underscores their importance in maintaining cellular communication and response to external stimuli, making Gβ (H-1) monoclonal antibody a valuable tool for researchers studying these critical mechanisms.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

G_β Antibody (H-1) References:

1. Different beta-subunits determine G-protein interaction with transmembrane receptors.  |  Kleuss, C., et al. 1992. Nature. 358: 424-6. PMID: 1322501
2. Activation of cytosolic phosphoinositide phospholipase C by G-protein beta gamma subunits.  |  Blank, JL., et al. 1992. J Biol Chem. 267: 23069-75. PMID: 1331076
3. Selective tissue distribution of G protein gamma subunits, including a new form of the gamma subunits identified by cDNA cloning.  |  Cali, JJ., et al. 1992. J Biol Chem. 267: 24023-7. PMID: 1385432
4. G protein beta gamma subunits synthesized in Sf9 cells. Functional characterization and the significance of prenylation of gamma.  |  Iñiguez-Lluhi, JA., et al. 1992. J Biol Chem. 267: 23409-17. PMID: 1429682
5. Diversity among the beta subunits of heterotrimeric GTP-binding proteins: characterization of a novel beta-subunit cDNA.  |  von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
6. Diversity of G proteins in signal transduction.  |  Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
7. G protein diversity is increased by associations with a variety of gamma subunits.  |  Gautam, N., et al. 1990. Proc Natl Acad Sci U S A. 87: 7973-7. PMID: 2122451
8. Inter-relationships between the heterotrimeric Gβ subunit AGB1, the receptor-like kinase FERONIA, and RALF1 in salinity response.  |  Yu, Y. and Assmann, SM. 2018. Plant Cell Environ. 41: 2475-2489. PMID: 29907954
9. Structural mechanism of calcium-mediated hormone recognition and Gβ interaction by the human melanocortin-1 receptor.  |  Ma, S., et al. 2021. Cell Res. 31: 1061-1071. PMID: 34453129
10. Alcohol self-administration and nicotine withdrawal alter biomarkers of stress and inflammation and prefrontal cortex changes in Gβ subunits.  |  Cruz, B., et al. 2023. Am J Drug Alcohol Abuse. 49: 321-332. PMID: 36206520
11. Selectivity in signal transduction determined by gamma subunits of heterotrimeric G proteins.  |  Kleuss, C., et al. 1993. Science. 259: 832-4. PMID: 8094261
12. Structural elements of G alpha subunits that interact with G beta gamma, receptors, and effectors.  |  Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-G_β Antibody (H-1) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-G_β Antibody (H-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

G_β Antibody (H-1) Specifications

Alternate Product Name: pan G-protein beta

Product Clone Name: H-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 302-340

Epitope Target: G_β

Epitope Species: mouse

Product Reactivity/Detection: Gβ1, Gβ2, Gβ3 and Gβ4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for G_β Antibody (H-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin