G_{β 5} Antibody (C-6): sc-515379

Gβ 5 Antibody (C-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects G beta 5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). G beta 5 (C-6) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. G beta 5 protein plays a crucial role in regulating G protein signaling pathways, particularly in the central nervous system where G beta 5 is highly expressed. G beta 5 protein is unique among beta subunits due to forming stable complexes with RGS (regulator of G protein signaling) proteins, which enhances GTPase activity of G alpha subunits, accelerating G protein signaling termination. This interaction maintains balanced signaling pathways controlling neurotransmission and hormone signaling. G beta 5′s distinct structure, including a long C-terminal tail, enables specific interactions with RGS proteins, distinguishing G beta 5 from other beta subunits lacking this feature. Understanding G beta 5′s function and interactions helps explain G beta 5′s role in health and disease, particularly in neurodegenerative disorders and metabolic diseases where G protein signaling becomes disrupted.

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G_{β 5} Antibody (C-6) References:

1. Genomic characterization of the human heterotrimeric G protein alpha, beta, and gamma subunit genes.  |  Hurowitz, EH., et al. 2000. DNA Res. 7: 111-20. PMID: 10819326
2. Different beta-subunits determine G-protein interaction with transmembrane receptors.  |  Kleuss, C., et al. 1992. Nature. 358: 424-6. PMID: 1322501
3. Activation of cytosolic phosphoinositide phospholipase C by G-protein beta gamma subunits.  |  Blank, JL., et al. 1992. J Biol Chem. 267: 23069-75. PMID: 1331076
4. Absence of the RGS9.Gbeta5 GTPase-activating complex in photoreceptors of the R9AP knockout mouse.  |  Keresztes, G., et al. 2004. J Biol Chem. 279: 1581-4. PMID: 14625292
5. Diversity among the beta subunits of heterotrimeric GTP-binding proteins: characterization of a novel beta-subunit cDNA.  |  von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
6. Gbeta5 is required for normal light responses and morphology of retinal ON-bipolar cells.  |  Rao, A., et al. 2007. J Neurosci. 27: 14199-204. PMID: 18094259
7. Diversity of G proteins in signal transduction.  |  Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
8. G protein diversity is increased by associations with a variety of gamma subunits.  |  Gautam, N., et al. 1990. Proc Natl Acad Sci U S A. 87: 7973-7. PMID: 2122451
9. The complex of G protein regulator RGS9-2 and Gβ(5) controls sensitization and signaling kinetics of type 5 adenylyl cyclase in the striatum.  |  Xie, K., et al. 2012. Sci Signal. 5: ra63. PMID: 22932702
10. The Gβ5 protein regulates sensitivity to TRAIL-induced cell death in colon carcinoma.  |  Fuchs, D., et al. 2015. Oncogene. 34: 2753-63. PMID: 25043307
11. Cellular and Subcellular Localization of the RGS7/Gβ5/R7BP Complex in the Cerebellar Cortex.  |  Aguado, C., et al. 2016. Front Neuroanat. 10: 114. PMID: 27965545
12. Chromosomal localization of genes encoding guanine nucleotide-binding protein subunits in mouse and human.  |  Blatt, C., et al. 1988. Proc Natl Acad Sci U S A. 85: 7642-6. PMID: 2902634

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-G_{β 5} Antibody (C-6) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-G_{β 5} Antibody (C-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

G_{β 5} Antibody (C-6) Specifications

Alternate Product Name: G protein subunit beta 5

Product Clone Name: C-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 27-51

Epitope Target: G_{β 5}

Epitope Species: human

Product Reactivity/Detection: G_{β 5}

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for G_{β 5} Antibody (C-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred G_β antibody is G_β Antibody (H-1): sc-166123.