G_{β 3} Antibody (G-5): sc-393908

Gβ 3 Antibody (G-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects the G beta 3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Gβ 3 (G-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. G beta 3 protein plays a crucial role in the function of heterotrimeric G proteins, which are essential for transmitting signals from cell surface receptors to intracellular effectors. G beta 3 proteins are involved in a wide array of physiological processes, including sensory perception, hormone signaling, and neurotransmission. G beta 3 subunit interacts with G protein alpha subunits and certain receptors, thereby modulating their activity and influencing downstream signaling pathways. This regulatory function is vital for maintaining cellular homeostasis and responding appropriately to external stimuli. In mammals, G protein family consists of multiple subunits, with beta subunits, including G beta 3, being integral to the overall signaling mechanism, highlighting their importance in both health and disease.

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G_{β 3} Antibody (G-5) References:

1. Genomic characterization of the human heterotrimeric G protein alpha, beta, and gamma subunit genes.  |  Hurowitz, EH., et al. 2000. DNA Res. 7: 111-20. PMID: 10819326
2. Effect of the (C825T) Gbeta(3) polymorphism on adrenoceptor-mediated lipolysis in human fat cells.  |  Rydén, M., et al. 2002. Diabetes. 51: 1601-8. PMID: 11978662
3. Different beta-subunits determine G-protein interaction with transmembrane receptors.  |  Kleuss, C., et al. 1992. Nature. 358: 424-6. PMID: 1322501
4. Activation of cytosolic phosphoinositide phospholipase C by G-protein beta gamma subunits.  |  Blank, JL., et al. 1992. J Biol Chem. 267: 23069-75. PMID: 1331076
5. Diversity among the beta subunits of heterotrimeric GTP-binding proteins: characterization of a novel beta-subunit cDNA.  |  von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
6. Diversity of G proteins in signal transduction.  |  Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
7. Gbeta3 forms distinct dimers with specific Ggamma subunits and preferentially activates the beta3 isoform of phospholipase C.  |  Poon, LS., et al. 2009. Cell Signal. 21: 737-44. PMID: 19168127
8. G protein diversity is increased by associations with a variety of gamma subunits.  |  Gautam, N., et al. 1990. Proc Natl Acad Sci U S A. 87: 7973-7. PMID: 2122451
9. Gβ3 is required for normal light ON responses and synaptic maintenance.  |  Dhingra, A., et al. 2012. J Neurosci. 32: 11343-55. PMID: 22895717
10. Hypertension-associated C825T polymorphism impairs the function of Gβ3 to target GRK2 ubiquitination.  |  Zha, Z., et al. 2016. Cell Discov. 2: 16005. PMID: 27462452
11. Chromosomal localization of genes encoding guanine nucleotide-binding protein subunits in mouse and human.  |  Blatt, C., et al. 1988. Proc Natl Acad Sci U S A. 85: 7642-6. PMID: 2902634
12. The Gβ1 and Gβ3 Subunits Differentially Regulate Rat Vascular Kv7 Channels.  |  Greenwood, IA. and Stott, JB. 2019. Front Physiol. 10: 1573. PMID: 31992990

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-G_{β 3} Antibody (G-5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-G_{β 3} Antibody (G-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

G_{β 3} Antibody (G-5) Specifications

Alternate Product Name: G protein subunit beta 3

Product Clone Name: G-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 25-52

Epitope Target: G_{β 3}

Epitope Species: human

Product Reactivity/Detection: Gβ 3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for G_{β 3} Antibody (G-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred G_β antibody is G_β Antibody (H-1): sc-166123.