GATAD2A Antibody (G-9): sc-514987
- GATAD2A Antibody (G-9) is a mouse monoclonal IgG1 κ GATAD2A antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 186-275 mapping within an internal region of GATAD2A of human origin
- GATAD2A Antibody (G-9) is recommended for detection of GATAD2A of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-GATAD2A Antibody (G-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GATAD2A Antibody (G-9) for WB applications. These reagents are now offered in bundles with GATAD2A Antibody (G-9) (see ordering information below).
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GATAD2A Antibody (G-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects GATAD2A protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-GATAD2A antibody (G-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GATAD2A, also known as p66α, is a highly conserved protein that plays a crucial role in development and is ubiquitously expressed across different tissues. GATAD2A protein is a key component of the NuRD (nucleosome remodeling and histone deacetylation) complex, which is essential for ATP-dependent chromatin remodeling and histone deacetylation activities. GATAD2A contains a GATA-type zinc finger domain and interacts with other proteins such as MBD2 and MBD3, facilitating the repression of transcription by enhancing the interaction with histone tails. Notably, GATAD2A′s functional repressor activity is dependent on post-translational modifications, specifically SUMOylation at Lys-30 and Lys-487, which are critical for its role in gene regulation. GATAD2A′s ability to modulate chromatin structure and gene expression underscores its importance in cellular processes and development, making GATAD2A (G-9) monoclonal antibody a valuable tool for researchers studying these mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GATAD2A Antibody (G-9) References:
- Two highly related p66 proteins comprise a new family of potent transcriptional repressors interacting with MBD2 and MBD3. | Brackertz, M., et al. 2002. J Biol Chem. 277: 40958-66. PMID: 12183469
- Use of MEDUSA-based data analysis and capillary HPLC-ion-trap mass spectrometry to examine complex immunoaffinity extracts of RBAp48. | Gururaja, T., et al. 2002. J Proteome Res. 1: 253-61. PMID: 12645902
- Developmental roles of the Mi-2/NURD-associated protein p66 in Drosophila. | Kon, C., et al. 2005. Genetics. 169: 2087-100. PMID: 15695365
- MBD3L2 interacts with MBD3 and components of the NuRD complex and can oppose MBD2-MeCP1-mediated methylation silencing. | Jin, SG., et al. 2005. J Biol Chem. 280: 12700-9. PMID: 15701600
- p66alpha and p66beta of the Mi-2/NuRD complex mediate MBD2 and histone interaction. | Brackertz, M., et al. 2006. Nucleic Acids Res. 34: 397-406. PMID: 16415179
- SUMO modification enhances p66-mediated transcriptional repression of the Mi-2/NuRD complex. | Gong, Z., et al. 2006. Mol Cell Biol. 26: 4519-28. PMID: 16738318
- Mutants in the mouse NuRD/Mi2 component P66alpha are embryonic lethal. | Marino, S. and Nusse, R. 2007. PLoS One. 2: e519. PMID: 17565372
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GATAD2A Antibody (G-9) | sc-514987 | 200 µg/ml | $316.00 | |||
GATAD2A Antibody (G-9): m-IgG Fc BP-HRP Bundle | sc-531256 | 200 µg Ab; 10 µg BP | $354.00 | |||
GATAD2A Antibody (G-9): m-IgGκ BP-HRP Bundle | sc-524892 | 200 µg Ab, 40 µg BP | $354.00 | |||
GATAD2A Antibody (G-9) AC | sc-514987 AC | 500 µg/ml, 25% agarose | $416.00 | |||
GATAD2A Antibody (G-9) HRP | sc-514987 HRP | 200 µg/ml | $316.00 | |||
GATAD2A Antibody (G-9) FITC | sc-514987 FITC | 200 µg/ml | $330.00 | |||
GATAD2A Antibody (G-9) PE | sc-514987 PE | 200 µg/ml | $343.00 | |||
GATAD2A Antibody (G-9) Alexa Fluor® 488 | sc-514987 AF488 | 200 µg/ml | $357.00 | |||
GATAD2A Antibody (G-9) Alexa Fluor® 546 | sc-514987 AF546 | 200 µg/ml | $357.00 | |||
GATAD2A Antibody (G-9) Alexa Fluor® 594 | sc-514987 AF594 | 200 µg/ml | $357.00 | |||
GATAD2A Antibody (G-9) Alexa Fluor® 647 | sc-514987 AF647 | 200 µg/ml | $357.00 | |||
GATAD2A Antibody (G-9) Alexa Fluor® 680 | sc-514987 AF680 | 200 µg/ml | $357.00 | |||
GATAD2A Antibody (G-9) Alexa Fluor® 790 | sc-514987 AF790 | 200 µg/ml | $357.00 |