Anti-GATA-3 Antibody (HG3-31) is a mouse monoclonal IgG1 κ GATA-3 antibody, cited in 408 publications, provided at 200 µg/ml
raised against recombinant GATA-3 of human origin
Anti-GATA-3 Antibody (HG3-31) is recommended for detection of GATA-3 of mouse, rat, human and avian origin by WB, IP, IF and IHC(P)
Anti-GATA-3 Antibody (HG3-31) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
TransCruz reagent for Gel Supershift and ChIP applications (sc-268 X, 200 µg/0.1 ml)
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GATA-3 (HG3-31): sc-268.
m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GATA-3 Antibody (HG3-31) for WB and IHC(P) applications. These reagents are now offered in bundles with GATA-3 Antibody (HG3-31) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
GATA-3 Antibody (HG3-31) is a high quality monoclonal GATA-3 antibody (also designated GATA3 antibody or GATA binding protein 3 antibody) suitable for the detection of the GATA-3 protein of mouse, rat, human and avian origin. GATA-3 Antibody (HG3-31) is available as both the non-conjugated anti-GATA-3 antibody form, as well as multiple conjugated forms of anti-GATA-3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Members of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence. GATA-1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes. It is an essential component in the generation of the erythroid lineage. GATA-2 is expressed in embryonic brain and liver, HeLa and endothelial cells, as well as in erythroid cells. Studies with a modified GATA consensus sequence, AGATCTTA, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence. This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1. GATA-3 is highly expressed in T lymphocytes and displays nuclear staining when detected by the GATA-3 Antibody (HG3-31) in immunostaining applications. GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors. Both GATA-4 and GATA-6 are found in heart, pancreas and ovary; lung and liver tissues exhibit GATA-6, but not GATA-4 expression. GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart. Although expression patterns of the various GATA transcription factors may overlap, it is not yet apparent how the GATA factors are able to discriminate in binding their appropriate target sites.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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