Anti-GATA-3 Antibody (HG3-31) recommended for detection of GATA-3 of mouse, rat, human and avian origin by WB, IP, IF and IHC(P)

Anti-GATA-3 Antibody (HG3-31): sc-268

Anti-GATA-3 Antibody (HG3-31) is rated 4.1 out of 5 by 23.
  • y_2022, m_5, d_19, h_21
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.27
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_23
  • loc_en_US, sid_268, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 113ms
  • REVIEWS, PRODUCT
loading
5
13
4
6
3
1
2
0
1
3

Datasheets

    • Anti-GATA-3 Antibody (HG3-31) is a mouse monoclonal IgG1 κ GATA-3 antibody, cited in 408 publications, provided at 200 µg/ml
    • raised against recombinant GATA-3 of human origin
    • Anti-GATA-3 Antibody (HG3-31) is recommended for detection of GATA-3 of mouse, rat, human and avian origin by WB, IP, IF and IHC(P)
    • Anti-GATA-3 Antibody (HG3-31) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-268 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GATA-3 (HG3-31): sc-268.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GATA-3 Antibody (HG3-31) for WB and IHC(P) applications. These reagents are now offered in bundles with GATA-3 Antibody (HG3-31) (see ordering information below).
loading

What is the region of the amino acids (i.e. N-terminus or C-terminus?) in the recombinant human GATA-3 raised for this monoclonal antibody?

Asked by: Jay1980
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-11-30

We bought this antibody, GATA-3 Antibody (HG3-31): sc-268, and did IF on the sections ( 5 um) of mouse breast tumors after antigen unmasking (PH 6, pressure cooking 2 min). However, the location of GATA3 is in the cytoplasm, not in the nuclei. Why?

Asked by: Mmcc
Thank you for your question. Please contact our Technical Service Department for help with troubleshooting the antibody. Please call us toll-free at (800) 457-3801 option 2, or use the live chat on our website.
Answered by: Technical Support
Date published: 2018-11-22

Does the HG3-31 antibody show significant cross-reactivity with the other GATA factors (that is GATA-1, GATA-2, GATA-4, GATA-5 or GATA-6)?

Asked by: wliam
Thank you for your question. This mouse monoclonal, GATA-3 Antibody (HG3-31): sc-268, is specific for GATA-3 and does not cross-react with other GATA factors. The antibody is extremely well characterized, and has been cited in the literature over 300 times. If you would be interested in testing a 10 µg sample of this antibody, for a limited time, we are offering a sample size. Please contact our Technical Service Department either by phone at (800) 457-3801 option 2, email [email protected], or use our live chat!
Answered by: Technical Support
Date published: 2018-09-10

may i ask that this antibody can detect GATA3 in pig?

Asked by: leseichi
Thank you for your question. We have not tested our mouse monoclonal, GATA-3 Antibody (HG3-31): sc-268, in pig. However, the antibody is raised against a recombinant protein immunogen that shares 94% sequence identity with the published protein for GATA-3 in pig (NP_001038032). Based on this sequence identity, we would expect this antibody to cross-react with GATA-3 in pig. If you are interested in testing a free sample of this antibody, please contact our Technical Service Department. As part of a limited time promotion, we are offering a free 10µg sample of our mouse monoclonal, GATA-3 Antibody (HG3-31): sc-268. Please call our Asian Support office toll-free at 800-820-8626, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-02-01

Dilution and protocol to use on Dako Autostainer Link 48? Thanks

Asked by: joann
Thank you for your question. Our recommended immunohistochemistry protocol for using our monoclonal, GATA-3 Antibody (HG3-31): sc-268, is provided below. We have not used this antibody in combination with the Dako Autostainer Link 48 system, so we are unable to provide you any specific recommendations. However, this antibody has been validated for IHC on formalin-fixed paraffin-embedded tissue sections so it should be compatible. You may want to contact the other supplier to see if they can offer any specific recommendations for antibody usage with their equipment. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-01-25

How reliable does the HG3-31 perform in mouse tissues?

Asked by: Professor Griffin
Thank you for your question. The HG3-31 clone is well established for mouse on mouse histological detection; PMID 19661437, PMID 17359917, PMID 17237387.
Answered by: Technical Support
Date published: 2016-12-20
  • y_2022, m_5, d_19, h_21CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.27
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_6
  • loc_en_US, sid_268, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 128ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from Not working Cannot get a specific band and got multiple unspecific bands.
Date published: 2020-10-08
Rated 1 out of 5 by from not working I purchased this ab two weeks ago since was one of the best rated for GATA3. I tried BSA and MILK blocking, 1 to 200 dilution and increasing concentrations of MCF7 cell lysates. It did not work so far
Date published: 2018-10-29
Rated 4 out of 5 by from anti GATA3 sc-268 anti GATA3 sc-268 , dilution 1/500 in human breast cancer cells
Date published: 2018-10-19
Rated 4 out of 5 by from Strong band Strong band seen in WB using GATA-3 in whole cell lysate.
Date published: 2017-10-10
Rated 5 out of 5 by from GATA-3 I used the antibody for immunofluroscence staining and it worked excellent
Date published: 2017-09-22
Rated 4 out of 5 by from GATA-3 wb is okay WB: 1:1000, mouse spleen tissue, 5% BSA block, antibody duluting with 5%BSA in TBST buffer. WB, two bind attached photos
Date published: 2017-09-02
Rated 4 out of 5 by from Good antibody The antibody works great for westernblotting using mouse brain tissue at 1:500 dilution.
Date published: 2017-07-24
Rated 5 out of 5 by from western blot I used it to do western blot .It have signal in Hela cell line at 1:500.
Date published: 2017-05-31
  • y_2022, m_5, d_19, h_21
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.27
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_23
  • loc_en_US, sid_268, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 17ms
  • REVIEWS, PRODUCT