GARNL1 Antibody (F-1): sc-376633
- GARNL1 Antibody (F-1) is a mouse monoclonal IgG2b κ GARNL1 antibody provided at 200 µg/ml
- raised against amino acids 263-443 mapping near the N-terminus of GARNL1 of human origin
- GARNL1 Antibody (F-1) is recommended for detection of GARNL1 of human origin by WB, IP, IF and ELISA
- Anti-GARNL1 Antibody (F-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GARNL1 Antibody (F-1) for WB applications. These reagents are now offered in bundles with GARNL1 Antibody (F-1) (see ordering information below).
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GARNL1 Antibody (F-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects GARNL1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GARNL1 monoclonal antibody (F-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GARNL1, also known as GTPase activating Rap/Ran-GAP domain-like 1, TULIP1, or GRIPE, contains a Rap-GAP domain and plays a crucial role in cellular signaling pathways. GARNL1 is primarily expressed during embryogenesis, with expression levels decreasing as development progresses, ultimately persisting at high levels only in neurons of the adult brain. GARNL1 is localized in the cytoplasm, where GARNL1 regulates GTP hydrolysis of proteins such as Ran and Rap, which are essential for various cellular processes, including cell division and intracellular transport. GARNL1 is imported into the nucleus through dimerization with E12, and GARNL1 interacts with the HLH region of E12, potentially functioning to negatively regulate the transcription of E12-dependent downstream target genes. This interaction highlights GARNL1′s importance in modulating gene expression during development. In the adult brain, GARNL1′s activity diverges from E12-related functions, suggesting distinct roles in mature neural tissue, thereby underscoring GARNL1′s significance in both developmental and adult cellular contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GARNL1 Antibody (F-1) References:
- Cloning and characterization of GRIPE, a novel interacting partner of the transcription factor E12 in developing mouse forebrain. | Heng, JI. and Tan, SS. 2002. J Biol Chem. 277: 43152-9. PMID: 12200424
- Cloning, genomic structure, and expression profiles of TULIP1 (GARNL1), a brain-expressed candidate gene for 14q13-linked neurological phenotypes, and its murine homologue. | Schwarzbraun, T., et al. 2004. Genomics. 84: 577-86. PMID: 15498464
- Expression of 25 high egg production related transcripts that identified from hypothalamus and pituitary gland in red-feather Taiwan country chickens. | Chen, LR., et al. 2007. Anim Reprod Sci. 100: 172-85. PMID: 16919900
- Laying traits and underlying transcripts, expressed in the hypothalamus and pituitary gland, that were associated with egg production variability in chickens. | Chen, CF., et al. 2007. Theriogenology. 68: 1305-15. PMID: 17931698
- Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. | Nagase, T., et al. 1998. DNA Res. 5: 277-86. PMID: 9872452
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GARNL1 Antibody (F-1) | sc-376633 | 200 µg/ml | $322.00 | |||
GARNL1 Antibody (F-1): m-IgG Fc BP-HRP Bundle | sc-529911 | 200 µg Ab; 10 µg BP | $361.00 | |||
GARNL1 Antibody (F-1): m-IgGκ BP-HRP Bundle | sc-523027 | 200 µg Ab, 40 µg BP | $361.00 | |||
GARNL1 Antibody (F-1): m-IgG2b BP-HRP Bundle | sc-550149 | 200 µg Ab; 10 µg BP | $361.00 | |||
GARNL1 Antibody (F-1) AC | sc-376633 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GARNL1 Antibody (F-1) HRP | sc-376633 HRP | 200 µg/ml | $322.00 | |||
GARNL1 Antibody (F-1) FITC | sc-376633 FITC | 200 µg/ml | $336.00 | |||
GARNL1 Antibody (F-1) PE | sc-376633 PE | 200 µg/ml | $349.00 | |||
GARNL1 Antibody (F-1) Alexa Fluor® 488 | sc-376633 AF488 | 200 µg/ml | $364.00 | |||
GARNL1 Antibody (F-1) Alexa Fluor® 546 | sc-376633 AF546 | 200 µg/ml | $364.00 | |||
GARNL1 Antibody (F-1) Alexa Fluor® 594 | sc-376633 AF594 | 200 µg/ml | $364.00 | |||
GARNL1 Antibody (F-1) Alexa Fluor® 647 | sc-376633 AF647 | 200 µg/ml | $364.00 | |||
GARNL1 Antibody (F-1) Alexa Fluor® 680 | sc-376633 AF680 | 200 µg/ml | $364.00 | |||
GARNL1 Antibody (F-1) Alexa Fluor® 790 | sc-376633 AF790 | 200 µg/ml | $364.00 |