GAPT Antibody (H-8): sc-398271
- GAPT Antibody (H-8) is a mouse monoclonal IgG2a κ GAPT antibody provided at 200 µg/ml
- raised against amino acids 84-135 mapping within an internal region of GAPT of human origin
- GAPT Antibody (H-8) is recommended for detection of GAPT of human origin by WB, IP, IF and ELISA
- Anti-GAPT Antibody (H-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GAPT Antibody (H-8) for WB applications. These reagents are now offered in bundles with GAPT Antibody (H-8) (see ordering information below).
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GAPT Antibody (H-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects GAPT protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GAPT (H-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GAPT protein, also known as growth factor receptor-bound protein 2-binding adapter protein, transmembrane, or C5orf29, is a 157 amino acid single-pass membrane protein that plays a crucial role in regulating B-cell proliferation. GAPT functions as a negative regulator after B-cell receptor stimulation, which is vital for maintaining B-cell marginal zone integrity and preventing excessive proliferation that could lead to malignancies. GAPT is known to interact with GRB2 protein, and GAPT expression is predominantly observed in peripheral blood lymphocytes and spleen, with lower levels detected in thymus. GAPT gene is conserved across several species, including chimpanzees, dogs, cows, mice, and rats, and is located on human chromosome 5q11.2. This chromosome is associated with various genetic disorders, including Cockayne syndrome and familial adenomatous polyposis, highlighting GAPT importance in both normal physiology and disease contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GAPT Antibody (H-8) References:
- Duodenal adenomatosis in familial adenomatous polyposis coli. A review of the literature and results from the Heidelberg Polyposis Register. | Kadmon, M., et al. 2001. Int J Colorectal Dis. 16: 63-75. PMID: 11355321
- The gene for Treacher Collins syndrome maps to the long arm of chromosome 5. | Dixon, MJ., et al. 1991. Am J Hum Genet. 49: 17-22. PMID: 1676560
- Cockayne syndrome exhibits dysregulation of p21 and other gene products that may be independent of transcription-coupled repair. | Cleaver, JE., et al. 2007. Neuroscience. 145: 1300-8. PMID: 17055654
- A new genomic mechanism leading to cri-du-chat syndrome. | South, ST., et al. 2006. Am J Med Genet A. 140: 2714-20. PMID: 17103439
- Redefining monosomy 5 by molecular cytogenetics in 23 patients with MDS/AML. | Herry, A., et al. 2007. Eur J Haematol. 78: 457-67. PMID: 17391336
- Somatic APC mosaicism: a frequent cause of familial adenomatous polyposis (FAP). | Aretz, S., et al. 2007. Hum Mutat. 28: 985-92. PMID: 17486639
- Telomerase reverse transcriptase haploinsufficiency and telomere length in individuals with 5p- syndrome. | Du, HY., et al. 2007. Aging Cell. 6: 689-97. PMID: 17875000
- Molecular mechanisms of skin aging: state of the art. | Makrantonaki, E. and Zouboulis, CC. 2007. Ann N Y Acad Sci. 1119: 40-50. PMID: 18056953
- Identification of a new transmembrane adaptor protein that constitutively binds Grb2 in B cells. | Liu, Y. and Zhang, W. 2008. J Leukoc Biol. 84: 842-51. PMID: 18559951
- A physical map of 15 loci on human chromosome 5q23-q33 by two-color fluorescence in situ hybridization. | Saltman, DL., et al. 1993. Genomics. 16: 726-32. PMID: 8325647
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GAPT Antibody (H-8) | sc-398271 | 200 µg/ml | $316.00 | |||
GAPT Antibody (H-8): m-IgG Fc BP-HRP Bundle | sc-530730 | 200 µg Ab; 10 µg BP | $354.00 | |||
GAPT Antibody (H-8): m-IgGκ BP-HRP Bundle | sc-524264 | 200 µg Ab, 40 µg BP | $354.00 | |||
GAPT Antibody (H-8): m-IgG2a BP-HRP Bundle | sc-547575 | 200 µg Ab; 10 µg BP | $354.00 | |||
GAPT Antibody (H-8) AC | sc-398271 AC | 500 µg/ml, 25% agarose | $416.00 | |||
GAPT Antibody (H-8) HRP | sc-398271 HRP | 200 µg/ml | $316.00 | |||
GAPT Antibody (H-8) FITC | sc-398271 FITC | 200 µg/ml | $330.00 | |||
GAPT Antibody (H-8) PE | sc-398271 PE | 200 µg/ml | $343.00 | |||
GAPT Antibody (H-8) Alexa Fluor® 488 | sc-398271 AF488 | 200 µg/ml | $357.00 | |||
GAPT Antibody (H-8) Alexa Fluor® 546 | sc-398271 AF546 | 200 µg/ml | $357.00 | |||
GAPT Antibody (H-8) Alexa Fluor® 594 | sc-398271 AF594 | 200 µg/ml | $357.00 | |||
GAPT Antibody (H-8) Alexa Fluor® 647 | sc-398271 AF647 | 200 µg/ml | $357.00 | |||
GAPT Antibody (H-8) Alexa Fluor® 680 | sc-398271 AF680 | 200 µg/ml | $357.00 | |||
GAPT Antibody (H-8) Alexa Fluor® 790 | sc-398271 AF790 | 200 µg/ml | $357.00 |