γPAK Antibody (H-4): sc-377194

γPAK Antibody (H-4) is a mouse monoclonal IgG2a antibody that detects γPAK in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). γPAK, a serine/threonine kinase, plays a crucial role in cellular signaling pathways by interacting with small GTPases Rac1 and Cdc42, which are pivotal in regulating various cellular processes, including cytoskeletal dynamics and cell motility. γPAK (H-4) antibody enables research into γPAK′s ability to modulate these GTPases′ activity; upon binding to their active GTP-bound forms, γPAK inhibits their intrinsic GTPase activity, leading to autophosphorylation at key sites such as Ser 141 and Thr 402. These phosphorylation events are critical for γPAK activation and subsequent dissociation from the Rac/Cdc42 complex, allowing γPAK to engage with downstream substrates like Mek kinase, which is integral to the JNK signaling pathway. anti-γPAK antibody (H-4) provides reliable detection and analysis of γPAK, facilitating research into cellular signaling and potential implications in various diseases.

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γPAK Antibody (H-4) References:

1. p21-activated protein kinase gamma-PAK is translocated and activated in response to hyperosmolarity. Implication of Cdc42 and phosphoinositide 3-kinase in a two-step mechanism for gamma-PAK activation.  |  Roig, J., et al. 2000. J Biol Chem. 275: 16933-40. PMID: 10748040
2. Cdc42-independent activation and translocation of the cytostatic p21-activated protein kinase gamma-PAK by sphingosine.  |  Roig, J., et al. 2001. FEBS Lett. 507: 195-9. PMID: 11684097
3. Phosphorylation of Mnk1 by caspase-activated Pak2/gamma-PAK inhibits phosphorylation and interaction of eIF4G with Mnk.  |  Orton, KC., et al. 2004. J Biol Chem. 279: 38649-57. PMID: 15234964
4. Involvement of phosphoinositide 3-kinase gamma, Rac, and PAK signaling in chemokine-induced macrophage migration.  |  Weiss-Haljiti, C., et al. 2004. J Biol Chem. 279: 43273-84. PMID: 15292195
5. A role for a CXCR2/phosphatidylinositol 3-kinase gamma signaling axis in acute and chronic vascular permeability.  |  Gavard, J., et al. 2009. Mol Cell Biol. 29: 2469-80. PMID: 19255141
6. p21-Activated kinase 3 (PAK3) protein regulates synaptic transmission through its interaction with the Nck2/Grb4 protein adaptor.  |  Thévenot, E., et al. 2011. J Biol Chem. 286: 40044-59. PMID: 21949127
7. The p21-activated kinase PAK3 forms heterodimers with PAK1 in brain implementing trans-regulation of PAK3 activity.  |  Combeau, G., et al. 2012. J Biol Chem. 287: 30084-96. PMID: 22815483
8. Inhibition of Gαi activity by Gβγ is mediated by PI 3-kinase-γ- and cSrc-dependent tyrosine phosphorylation of Gαi and recruitment of RGS12.  |  Huang, J., et al. 2014. Am J Physiol Gastrointest Liver Physiol. 306: G802-10. PMID: 24578342
9. Restored autophagy is protective against PAK3-induced cardiac dysfunction.  |  Ruiz-Velasco, A., et al. 2023. iScience. 26: 106970. PMID: 37324527
10. Myosin phosphorylation by human cdc42-dependent S6/H4 kinase/gammaPAK from placenta and lymphoid cells.  |  Ramos, E., et al. 1997. Recept Signal Transduct. 7: 99-110. PMID: 9392438

The preferred α/β/γPAK antibody is α/β/γPAK Antibody (D-8): sc-166174.