γPAK Antibody (G-10): sc-137208
- γPAK Antibody (G-10) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-41 at the N-terminus of γPAK of human origin
- recommended for detection of γPAK p62 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for γPAK Antibody (G-10) for WB applications. These reagents are now offered in bundles with γPAK Antibody (G-10) (see ordering information below).
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γPAK Antibody (G-10) is a mouse monoclonal IgG1 antibody that detects γPAK in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). γPAK, a serine/threonine kinase, plays a crucial role in cellular signaling pathways by interacting with the small GTPases Rac1 and Cdc42, which are pivotal in regulating various cellular processes, including cytoskeletal dynamics and cell motility. γPAK modulates the activity of these GTPases; upon binding to their active GTP-bound forms, γPAK inhibits their intrinsic GTPase activity, leading to autophosphorylation at key sites such as Ser 141 and Thr 402. This phosphorylation is essential for γPAK activation and subsequent dissociation from the Rac/Cdc42 complex, allowing γPAK to engage with downstream substrates like Mek kinase, which is integral to the JNK signaling pathway. Anti-γPAK antibody (G-10) serves as an invaluable tool for researchers studying the intricate signaling networks that govern cell behavior and response to external stimuli.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
γPAK Antibody (G-10) References:
- Multisite autophosphorylation of p21-activated protein kinase gamma-PAK as a function of activation. | Gatti, A., et al. 1999. J Biol Chem. 274: 8022-8. PMID: 10075701
- p21-activated protein kinase gamma-PAK is activated by ionizing radiation and other DNA-damaging agents. Similarities and differences to alpha-PAK. | Roig, J. and Traugh, JA. 1999. J Biol Chem. 274: 31119-22. PMID: 10531298
- Substrates enhance autophosphorylation and activation of p21-activated protein kinase gamma-PAK in the absence of activation loop phosphorylation. | Jakobi, R., et al. 2000. Eur J Biochem. 267: 4414-21. PMID: 10880965
- p21-activated protein kinase gamma-PAK suppresses programmed cell death of BALB3T3 fibroblasts. | Jakobi, R., et al. 2001. J Biol Chem. 276: 16624-34. PMID: 11278362
- Cytostatic p21 G protein-activated protein kinase gamma-PAK. | Roig, J. and Traugh, JA. 2001. Vitam Horm. 62: 167-98. PMID: 11345898
- p21-activated protein kinase gamma-PAK in pituitary secretory granules phosphorylates prolactin. | Tuazon, PT., et al. 2002. FEBS Lett. 515: 84-8. PMID: 11943200
- Localization of p21-activated protein kinase gamma-PAK/Pak2 in the endoplasmic reticulum is required for induction of cytostasis. | Huang, Z., et al. 2003. J Biol Chem. 278: 13101-9. PMID: 12560339
- Determinants for substrate phosphorylation by p21-activated protein kinase (gamma-PAK). | Tuazon, PT., et al. 1997. Biochemistry. 36: 16059-64. PMID: 9405039
- Cleavage and activation of p21-activated protein kinase gamma-PAK by CPP32 (caspase 3). Effects of autophosphorylation on activity. | Walter, BN., et al. 1998. J Biol Chem. 273: 28733-9. PMID: 9786869
- Autophosphorylation and protein kinase activity of p21-activated protein kinase gamma-PAK are differentially affected by magnesium and manganese. | Tuazon, PT., et al. 1998. Biochemistry. 37: 17024-9. PMID: 9836597
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
γPAK Antibody (G-10) | sc-137208 | 200 µg/ml | $316.00 | |||
γPAK Antibody (G-10): m-IgG Fc BP-HRP Bundle | sc-537422 | 200 µg Ab; 10 µg BP | $354.00 | |||
γPAK Antibody (G-10): m-IgGκ BP-HRP Bundle | sc-534680 | 200 µg Ab; 40 µg BP | $354.00 | |||
γPAK Antibody (G-10): m-IgG1 BP-HRP Bundle | sc-545180 | 200 µg Ab; 20 µg BP | $354.00 | |||
γPAK (G-10) Neutralizing Peptide | sc-137208 P | 100 µg/0.5 ml | $68.00 |