γPAK Antibody (E-9): sc-373740

γPAK Antibody (E-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects γPAK p62 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-γPAK antibody (E-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. γPAK p62 is a serine/threonine kinase that plays a crucial role in cellular signaling pathways, particularly in regulating the JNK signaling cascade. γPAK p62 is located in the cytoplasm and interacts with active forms of Rac1 and Cdc42, which are small GTPases involved in various cellular processes, including cytoskeletal reorganization and cell migration. γPAK p62 interaction with these GTPases leads to phosphorylation of downstream substrates, such as MEK kinase, which subsequently activates the JNK pathway. This pathway is vital for cellular responses to stress and apoptosis, highlighting γPAK p62′s importance in maintaining cellular homeostasis and responding to external stimuli. γPAK monoclonal antibody (E-9) enables researchers to gain insights into the regulatory mechanisms of γPAK p62 and its implications in various biological processes and diseases.

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γPAK Antibody (E-9) References:

1. Functional interaction between c-Abl and the p21-activated protein kinase gamma-PAK.  |  Roig, J., et al. 2000. Proc Natl Acad Sci U S A. 97: 14346-51. PMID: 11121037
2. Phosphorylation of the activation loop of gamma p21-activated kinase (gamma-Pak) and related kinases (MSTs) in normal and stressed neutrophils.  |  Lian, JP., et al. 2001. J Immunol. 166: 6349-57. PMID: 11342659
3. Localization of p21-activated protein kinase gamma-PAK/Pak2 in the endoplasmic reticulum is required for induction of cytostasis.  |  Huang, Z., et al. 2003. J Biol Chem. 278: 13101-9. PMID: 12560339
4. Activation of Syk protein tyrosine kinase in response to osmotic stress requires interaction with p21-activated protein kinase Pak2/gamma-PAK.  |  Miah, SM., et al. 2004. Mol Cell Biol. 24: 71-83. PMID: 14673144
5. Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42.  |  Shinjo, K., et al. 1990. Proc Natl Acad Sci U S A. 87: 9853-7. PMID: 2124704
6. rac, a novel ras-related family of proteins that are botulinum toxin substrates.  |  Didsbury, J., et al. 1989. J Biol Chem. 264: 16378-82. PMID: 2674130
7. The small GTP-binding proteins Rac1 and Cdc42 regulate the activity of the JNK/SAPK signaling pathway.  |  Coso, OA., et al. 1995. Cell. 81: 1137-46. PMID: 7600581
8. A novel serine kinase activated by rac1/CDC42Hs-dependent autophosphorylation is related to PAK65 and STE20.  |  Martin, GA., et al. 1995. EMBO J. 14: 1970-8. PMID: 7744004
9. Differential activation of ERK and JNK mitogen-activated protein kinases by Raf-1 and MEKK.  |  Minden, A., et al. 1994. Science. 266: 1719-23. PMID: 7992057
10. Activation of stress-activated protein kinase by MEKK1 phosphorylation of its activator SEK1.  |  Yan, M., et al. Nature. 372: 798-800. PMID: 7997270
11. A brain serine/threonine protein kinase activated by Cdc42 and Rac1.  |  Manser, E., et al. 1994. Nature. 367: 40-6. PMID: 8107774
12. Proteins regulating Ras and its relatives.  |  Boguski, MS. and McCormick, F. 1993. Nature. 366: 643-54. PMID: 8259209

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-γPAK Antibody (E-9) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-γPAK Antibody (E-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

γPAK Antibody (E-9) Specifications

Alternate Product Name: γPAK

Product Clone Name: E-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 497-522

Epitope Target: γPAK

Epitope Species: human

Product Reactivity/Detection: γPAK p62

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for γPAK Antibody (E-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred α/β/γPAK antibody is α/β/γPAK Antibody (D-8): sc-166174.