γ-GCSc Antibody (G-12): sc-166345

γ-GCSc Antibody (G-12) is a mouse monoclonal IgG1 antibody that detects γ-GCSc (γ-glutamylcysteine synthetase heavy subunit) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). γ-GCSc, encoded by the GCLC gene consisting of 16 exons, plays a crucial role in glutathione biosynthesis, the body′s primary antioxidant, by catalyzing the formation of γ-L-glutamate-L-cysteine, the first and rate-limiting step in this pathway. γ-GCSc is predominantly expressed in hemocytes, brain, liver, and kidney, where γ-GCSc forms a heterodimer with the regulatory subunit γ-GCSm, collectively known as γ-GCS. γ-GCSc functions extend beyond enzymatic activity as a key player in cellular responses to oxidative stress, with expression upregulated by various oxidants and heavy metals, including cadmium and methyl mercury. γ-GCSc transcription regulation involves complex interactions with proteins such as Yap1, which responds to hydrogen peroxide, and Cbf1, a DNA-binding protein that inhibits transcription. A His370Leu amino acid substitution in γ-GCSc has been linked to reduced enzymatic activity, resulting in hemolytic anemia and diminished glutathione levels in red blood cells, highlighting γ-GCSc′s critical role in maintaining cellular health and preventing oxidative damage. anti-γ-GCSc antibody (G-12) serves as an essential tool for researchers investigating glutathione biosynthesis mechanisms and their implications in various diseases.

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γ-GCSc Antibody (G-12) References:

1. Enhanced expression of pulmonary gamma-glutamylcysteine synthetase heavy subunit in rats exposed to cadmium aerosols.  |  Shukla, GS., et al. 2000. Toxicol Appl Pharmacol. 163: 249-59. PMID: 10702364
2. The influence of pro-longevity gene Gclc overexpression on the age-dependent changes in Drosophila transcriptome and biological functions.  |  Moskalev, A., et al. 2016. BMC Genomics. 17: 1046. PMID: 28105938
3. Polymorphism of Glutamate-Cysteine Ligase Subunit Catalytic (GCLC) Gene in Pulmonary Tuberculosis Patients.  |  Yuniastuti, A., et al. 2017. Pak J Biol Sci. 20: 397-402. PMID: 29023060
4. H1N1 influenza virus infection through NRF2-KEAP1-GCLC pathway induces ferroptosis in nasal mucosal epithelial cells.  |  Liu, C., et al. 2023. Free Radic Biol Med. 204: 226-242. PMID: 37146698
5. GGT1 Suppresses the Development of Ferroptosis and Autophagy in Mouse Retinal Ganglion Cell Through Targeting GCLC.  |  Xu, G., et al. 2023. Eye Brain. 15: 139-151. PMID: 38020723
6. SNORA56-mediated pseudouridylation of 28 S rRNA inhibits ferroptosis and promotes colorectal cancer proliferation by enhancing GCLC translation.  |  Xu, C., et al. 2023. J Exp Clin Cancer Res. 42: 331. PMID: 38049865
7. Gclc as a Marker for Injured Distal Nephron in Ischemia-Reperfusion Induced Acute Kidney Injury.  |  Li, Y., et al. 2024. J Inflamm Res. 17: 527-540. PMID: 38313210
8. Prevention of age-related truncation of γ-glutamylcysteine ligase catalytic subunit (GCLC) delays cataract formation.  |  Wei, Z., et al. 2024. Sci Adv. 10: eadl1088. PMID: 38669339
9. Implications of GCLC in prognosis and immunity of lung adenocarcinoma and multi-omics regulation mechanisms.  |  Huang, Z., et al. 2024. BMC Pulm Med. 24: 239. PMID: 38750474
10. Characterisation of gamma-glutamylcysteine synthetase-heavy subunit promoter: a critical role for AP-1.  |  Rahman, I., et al. 1998. FEBS Lett. 427: 129-33. PMID: 9613613

The preferred γ-GCSc antibody is γ-GCSc Antibody (H-5): sc-390811.