gamma-catenin Antibody (15F11) recommended for detection of γ-catenin of mouse, rat and human origin by WB, IP and IF

γ-catenin Antibody (15F11): sc-33634

γ-catenin Antibody (15F11) is rated 4.0 out of 5 by 1.
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Datasheets

    • γ-catenin Antibody (15F11) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant γ-catenin of chicken origin
    • recommended for detection of γ-catenin of mouse, rat and human origin by WB, IP and IF
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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How much sample do you recommend loading on the gel for Western blot detection using γ-catenin (15F11): sc-33634?

Asked by: DefinitelyNotMatt
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Positive western blot detection of Positive western blot detection of -catenin expression in HeLa whole cell lysate. -SCBT QC
Date published: 2014-07-17
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