gamma-catenin Antibody (A-6) recommended for detection of γ-catenin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

γ-catenin Antibody (A-6): sc-514115

γ-catenin Antibody (A-6) is rated 4.8 out of 5 by 5.
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Datasheets

    • Anti-γ-catenin Antibody (A-6) is a mouse monoclonal IgG2a (kappa light chain) γ-catenin antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 689-713 near the C-terminus of γ-catenin of human origin
    • Anti-gamma-catenin Antibody (A-6) is recommended for detection of γ-catenin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-gamma-catenin Antibody (A-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-514115 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of γ-catenin (A-6): sc-514115.
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Hi. I am interested in purchasing Anti-γ-Catenin Antibody for flow cytometry. Could you please let me know which of the two clones you are selling is preferable for this application? Thanks

Asked by: Elad
Thank you for your question. sc-514115 conjugated to Alexa Fluor is the only gamma catenin antibody we offer that is recommended for FCM.
Answered by: Tech Service
Date published: 2019-01-28

What application is the blocking peptide sc-514115 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Ab Biotechnology J. Excellent antibody This antibody works well for WB. Good signal was obtained using at 1:1000 dilution with 20 -25 μg of loaded proteins. Band at the expected size.
Date published: 2018-10-19
Rated 5 out of 5 by from sharp bands This antibody was tested on human aortic endothelial cells post various treatments. It gives specific band with no background.
Date published: 2017-09-14
Rated 5 out of 5 by from ottimo Ab Segnale perfetto. L'Ab è stato utilizzato in western blotting, con una diluizione di 1:2000, caricando 10-15 ug di proteine totali isolate da linee epiteliali umane.
Date published: 2017-07-19
Rated 4 out of 5 by from Faint bands for WB Produced faint but distinct bands on WB using MCF-7 cell lysates. Used the ab at 1:1000 dilution
Date published: 2017-07-03
Rated 5 out of 5 by from Great mono Great mono! Strong WB data in HeLa, A-431, HT-1080, H4, T-47D and MCF7 whole cell lysates. -SCBT QC
Date published: 2014-01-04
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