gamma-Actin Antibody (2-2) recommended for detection of γ-Actin of mouse, rat and human origin by WB, IP, IF and IHC(P)

γ-Actin Antibody (2-2): sc-65636

γ-Actin Antibody (2-2) is rated 4.7 out of 5 by 3.
  • y_2020, m_9, d_19, h_15
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_3
  • loc_en_US, sid_65636, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 93ms
  • REVIEWS, PRODUCT
5
2
4
1
3
0
2
0
1
0

Datasheets

    • γ-Actin Antibody (2-2) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised aginast cytoplasmic γ-Actin from brain tissue of bovine origin
    • recommended for detection of γ-Actin of mouse, rat and human origin by WB, IP, IF and IHC(P); also reactive with additional species, including bovine
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

Can γ-Actin (2-2): sc-65636 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Germaine
Thank you for your question. Yes, γ-Actin (2-2): sc-65636 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
  • y_2020, m_9, d_21, h_15CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_65636, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 148ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from great for wb This antibody is very great for WB. We always get single band.
Date published: 2017-01-06
Rated 4 out of 5 by from Works in paraffin Works in paraffin-embedded sections with citrate buffer retrieval. -SCBT QC
Date published: 2014-07-25
Rated 5 out of 5 by from Great for Westerns at 1:100 dilution Great for Westerns at 1:100 dilution, 1 hour incubation at room temperature. -SCBT QC
Date published: 2014-05-10
  • y_2020, m_9, d_19, h_15
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_3
  • loc_en_US, sid_65636, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 15ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.