gamma-Actin Antibody (1-17) recommended for detection of γ Actin of mouse, rat and human origin by WB, IP, IF and IHC(P)

γ-Actin Antibody (1-17): sc-65638

γ-Actin Antibody (1-17) is rated 4.7 out of 5 by 3.
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Datasheets

    • Anti-γ-Actin Antibody (1-17) is a mouse monoclonal IgG1 (kappa light chain) γ-Actin antibody provided at 200 µg/ml
    • raised aginast cytoplasmic γ-Actin from brain tissue of bovine origin
    • Anti-gamma-Actin Antibody (1-17) is recommended for detection of γ Actin of mouse, rat and human origin by WB, IP, IF and IHC(P); also reactive with additional species, including bovine
    • Anti-gamma-Actin Antibody (1-17) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of γ-Actin (1-17): sc-65638.

I am using γ-Actin (1-17): sc-65638 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Randy McDonald
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent antibody! Strong solid bands on the Western blot and clear specific staining on IHC. Great choice!
Date published: 2017-01-20
Rated 5 out of 5 by from Clean signal in Western with human and mouse Clean signal in Western with human and mouse whole cell lysates. -SCBT QC
Date published: 2014-03-30
Rated 4 out of 5 by from Obtained good immunoperoxidase staining of Obtained good immunoperoxidase staining of formalin fixed, paraffin-embedded human upper stomach tissue showing cytoplasmic and membrane staining of glandular cells. -SCBT QC
Date published: 2014-02-06
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