Gα i-1/GNAI1 Antibody (R4): sc-13533

Gα i-1 Antibody (R4) is a mouse monoclonal IgG2b kappa light chain antibody that detects Gα i-1 in mouse, rat, human, and bovine samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Gα i-1 antibody (R4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Gα i-1 plays a crucial role in cellular signaling as a member of the heterotrimeric G protein family, which transmits signals from cell surface receptors to intracellular effectors. Specifically, Gα i-1 is involved in inhibiting adenylate cyclase activity, leading to a decrease in cyclic AMP levels, which is vital for regulating various physiological processes such as neurotransmitter release, hormone secretion, and cell growth. Gα i-1 interaction with specific receptors and downstream effectors allows modulation of diverse signaling pathways, making Gα i-1 an important target for research in areas such as cardiovascular health, neurobiology, and cancer. Understanding Gα i-1 function and interactions can provide insights into the mechanisms of diseases and potential therapeutic targets.

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Gα i-1/GNAI1 Antibody (R4) References:

1. Immunoprecipitation of high-affinity, guanine nucleotide-sensitive, solubilized mu-opioid receptors from rat brain: coimmunoprecipitation of the G proteins G(alpha o), G(alpha i1), and G(alpha i3).  |  Chalecka-Franaszek, E., et al. 2000. J Neurochem. 74: 1068-78. PMID: 10693938
2. Conformational changes in the amino-terminal helix of the G protein alpha(i1) following dissociation from Gbetagamma subunit and activation.  |  Medkova, M., et al. 2002. Biochemistry. 41: 9962-72. PMID: 12146960
3. The myristoylated amino terminus of Galpha(i)(1) plays a critical role in the structure and function of Galpha(i)(1) subunits in solution.  |  Preininger, AM., et al. 2003. Biochemistry. 42: 7931-41. PMID: 12834345
4. Selective tissue distribution of G protein gamma subunits, including a new form of the gamma subunits identified by cDNA cloning.  |  Cali, JJ., et al. 1992. J Biol Chem. 267: 24023-7. PMID: 1385432
5. Diversity among the beta subunits of heterotrimeric GTP-binding proteins: characterization of a novel beta-subunit cDNA.  |  von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
6. Gustducin is a taste-cell-specific G protein closely related to the transducins.  |  McLaughlin, SK., et al. 1992. Nature. 357: 563-9. PMID: 1608467
7. Diversity of G proteins in signal transduction.  |  Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
8. GDP-GTP exchange processes of G{alpha}i1 protein are accelerated/decelerated depending on the type and the concentration of added detergents.  |  Kubota, M., et al. 2009. J Biochem. 146: 875-80. PMID: 19703944
9. Nucleobindin 1 is a calcium-regulated guanine nucleotide dissociation inhibitor of G{alpha}i1.  |  Kapoor, N., et al. 2010. J Biol Chem. 285: 31647-60. PMID: 20679342
10. Biochemical characterization of three stimulatory GTP-binding proteins. The large and small forms of Gs and the olfactory-specific G-protein, Golf.  |  Jones, DT., et al. 1990. J Biol Chem. 265: 2671-6. PMID: 2105931
11. The gene for the alpha i1 subunit of human guanine nucleotide binding protein maps near the cystic fibrosis locus.  |  Bloch, DB., et al. 1988. Am J Hum Genet. 42: 884-8. PMID: 3130752
12. Structural elements of G alpha subunits that interact with G beta gamma, receptors, and effectors.  |  Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Gα i-1/GNAI1 Antibody (R4) has 35 citations in a variety of scientific publications.

Primary antibodies like Anti-Gα i-1/GNAI1 Antibody (R4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Gα i-1/GNAI1 Antibody (R4) Specifications

Alternate Product Name: G protein subunit GNAI1

Product Clone Name: R4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: Gα i-1

Epitope Species: rat

Product Reactivity/Detection: G_{α i-1}

Species Reactivity/Detection: mouse, rat, human, bovine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 35 citations in various scientific publications

Available Conjugates/Forms for Gα i-1/GNAI1 Antibody (R4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin