Date published: 2026-4-24

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GALK1 Antibody (F-3): sc-398476

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Datasheets
  • GALK1 Antibody (F-3) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
  • raised against amino acids 41-259 mapping near the N-terminus of GALK1 of human origin
  • recommended for detection of GALK1 of human origin by WB, IP, IF and ELISA
  • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GALK1 Antibody (F-3) for WB applications. These reagents are now offered in bundles with GALK1 Antibody (F-3) (see ordering information below).
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    GALK1 Antibody (F-3) is a mouse monoclonal IgG2b antibody that detects galactose kinase (GALK1) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GALK1 plays a pivotal role in galactose metabolism, catalyzing the phosphorylation of galactose to galactose-1-phosphate, which is essential for proper sugar utilization in energy production and biosynthesis. GALK1 function extends beyond metabolism; defects in GALK1 can lead to galactosemia II, a serious autosomal recessive disorder that manifests in infancy with congenital cataracts, often appearing within the first two weeks of life. In adults, galactitol accumulation due to impaired GALK1 function can result in presenile cataracts, highlighting GALK1′s critical role in maintaining ocular health. Anti-GALK1 antibody (F-3) serves as an invaluable tool for researchers studying galactose metabolism and associated disorders, providing insights into underlying molecular mechanisms.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    GALK1 Antibody (F-3)

    sc-398476
    200 µg/ml
    $322.00

    GALK1 Antibody (F-3): m-IgGκ BP-HRP Bundle

    sc-536069
    200 µg Ab; 40 µg BP
    $361.00

    GALK1 Antibody (F-3): m-IgG2b BP-HRP Bundle

    sc-549510
    200 µg Ab; 10 µg BP
    $361.00

    How much sample do you recommend loading on the gel for Western blot detection using GALK1 (F-3): sc-398476?

    Asked by: Trav11
    We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 5 out of 5 by from Very good Western blot data of GALK1 expressionVery good Western blot data of GALK1 expression in Hep G2 and Y79 whole cell lysates and human liver tissue extract . -SCBT QC
    Date published: 2014-08-10
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    GALK1 Antibody (F-3) is rated 5.0 out of 5 by 1.
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