GALK1 Antibody (A-2): sc-393404
- GALK1 Antibody (A-2) is a mouse monoclonal IgG1 κ GALK1 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 18-43 at the N-terminus of GALK1 of human origin
- GALK1 Antibody (A-2) is recommended for detection of GALK1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-GALK1 Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GALK1 Antibody (A-2) for WB applications. These reagents are now offered in bundles with GALK1 Antibody (A-2) (see ordering information below).
QUICK LINKS
GALK1 Antibody (A-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects GALK1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GALK1 (A-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Galactose kinase (GALK1), also known as galactokinase, plays a vital role in the first step of the galactose metabolism pathway, converting galactose into galactose-1-phosphate, which is essential for energy production and the synthesis of biomolecules. This enzymatic activity is crucial because defects in the GALK1 gene can lead to galactosemia II, an autosomal recessive disorder that manifests in infancy with congenital cataracts, often appearing within the first two weeks of life. In adults, the accumulation of galactitol due to impaired GALK1 function can result in presenile cataracts, highlighting the importance of GALK1 in maintaining ocular health. Anti-GALK1 antibody (A-2) effectively detects and quantifies GALK1 across different species, making this reagent an invaluable tool for research into galactose metabolism and related disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GALK1 Antibody (A-2) References:
- Novel mutations in the GALK1 gene in patients with galactokinase deficiency. | Hunter, M., et al. 2001. Hum Mutat. 17: 77-8. PMID: 11139256
- A genetic factor for age-related cataract: identification and characterization of a novel galactokinase variant, 'Osaka,' in Asians. | Okano, Y., et al. 2001. Am J Hum Genet. 68: 1036-42. PMID: 11231902
- Functional analysis of disease-causing mutations in human galactokinase. | Timson, DJ. and Reece, RJ. 2003. Eur J Biochem. 270: 1767-74. PMID: 12694189
- Biochemical characterization of two GALK1 mutations in patients with galactokinase deficiency. | Sangiuolo, F., et al. 2004. Hum Mutat. 23: 396. PMID: 15024738
- Differential roles of the Leloir pathway enzymes and metabolites in defining galactose sensitivity in yeast. | Ross, KL., et al. 2004. Mol Genet Metab. 83: 103-16. PMID: 15464425
- Galactokinase: structure, function and role in type II galactosemia. | Holden, HM., et al. 2004. Cell Mol Life Sci. 61: 2471-84. PMID: 15526155
- Molecular structure of human galactokinase: implications for type II galactosemia. | Thoden, JB., et al. 2005. J Biol Chem. 280: 9662-70. PMID: 15590630
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GALK1 Antibody (A-2) | sc-393404 | 200 µg/ml | $322.00 | |||
GALK1 Antibody (A-2): m-IgG Fc BP-HRP Bundle | sc-530445 | 200 µg Ab; 10 µg BP | $361.00 | |||
GALK1 Antibody (A-2): m-IgGκ BP-HRP Bundle | sc-523932 | 200 µg Ab, 40 µg BP | $361.00 | |||
GALK1 Antibody (A-2): m-IgG1 BP-HRP Bundle | sc-544056 | 200 µg Ab; 20 µg BP | $361.00 | |||
GALK1 Antibody (A-2) AC | sc-393404 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GALK1 Antibody (A-2) HRP | sc-393404 HRP | 200 µg/ml | $322.00 | |||
GALK1 Antibody (A-2) FITC | sc-393404 FITC | 200 µg/ml | $336.00 | |||
GALK1 Antibody (A-2) PE | sc-393404 PE | 200 µg/ml | $349.00 | |||
GALK1 Antibody (A-2) Alexa Fluor® 488 | sc-393404 AF488 | 200 µg/ml | $364.00 | |||
GALK1 Antibody (A-2) Alexa Fluor® 546 | sc-393404 AF546 | 200 µg/ml | $364.00 | |||
GALK1 Antibody (A-2) Alexa Fluor® 594 | sc-393404 AF594 | 200 µg/ml | $364.00 | |||
GALK1 Antibody (A-2) Alexa Fluor® 647 | sc-393404 AF647 | 200 µg/ml | $364.00 | |||
GALK1 Antibody (A-2) Alexa Fluor® 680 | sc-393404 AF680 | 200 µg/ml | $364.00 | |||
GALK1 Antibody (A-2) Alexa Fluor® 790 | sc-393404 AF790 | 200 µg/ml | $364.00 | |||
GALK1 (A-2) Neutralizing Peptide | sc-393404 P | 100 µg/0.5 ml | $69.00 |