galectin-4 Antibody (C-6): sc-376263

Galectin-4 Antibody (C-6) is a mouse monoclonal IgG2a antibody that detects galectin-4 in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Galectin-4, also known as Gal-4, L36, or LGALS4, is a member of the galectin family, which consists of soluble β-galactoside-binding lectins that play crucial roles in various biological processes, including cell adhesion, tumor progression, and apoptosis. Anti-galectin-4 antibody (C-6) recognizes a protein composed of 323 amino acids featuring two homologous carbohydrate recognition domains, which are essential for binding to glycoproteins and glycolipids, thereby influencing cell-cell and cell-extracellular matrix interactions. Notably, galectin-4 is primarily expressed in epithelial cells of the colon, rectum, and intestine, and expression levels are closely associated with the malignant potential of human hepatocellular carcinoma. Galectin-4 is underexpressed in colorectal cancer, suggesting a potential role as a tumor suppressor, while upregulation in cells prone to peritoneal dissemination highlights significance in cancer metastasis. The ability of galectin-4 to modulate cellular interactions and differential regulation based on various physiological conditions make this protein critical for understanding tumor biology and developing therapeutic strategies.

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galectin-4 Antibody (C-6) References:

1. Fine specificity of domain-I of recombinant tandem-repeat-type galectin-4 from rat gastrointestinal tract (G4-N).  |  Wu, AM., et al. 2002. Biochem J. 367: 653-64. PMID: 12133001
2. Expression of galectin 4 in the rat small intestine during postnatal development.  |  Niepceron, E., et al. 2004. Biochimie. 86: 115-8. PMID: 15016449
3. Induced reactivity of intestinal CD4(+) T cells with an epithelial cell lectin, galectin-4, contributes to exacerbation of intestinal inflammation.  |  Hokama, A., et al. 2004. Immunity. 20: 681-93. PMID: 15189734
4. Effects of polyvalency of glycotopes and natural modifications of human blood group ABH/Lewis sugars at the Galbeta1-terminated core saccharides on the binding of domain-I of recombinant tandem-repeat-type galectin-4 from rat gastrointestinal tract (G4-N).  |  Wu, AM., et al. 2004. Biochimie. 86: 317-26. PMID: 15194236
5. Crystallization and preliminary X-ray diffraction analysis of mouse galectin-4 N-terminal carbohydrate recognition domain in complex with lactose.  |  Krejciríková, V., et al. 2008. Acta Crystallogr Sect F Struct Biol Cryst Commun. 64: 665-7. PMID: 18607104
6. Structure of the mouse galectin-4 N-terminal carbohydrate-recognition domain reveals the mechanism of oligosaccharide recognition.  |  Krejčiříková, V., et al. 2011. Acta Crystallogr D Biol Crystallogr. 67: 204-11. PMID: 21358051
7. Expression patterns suggest that despite considerable functional redundancy, galectin-4 and -6 play distinct roles in normal and damaged mouse digestive tract.  |  Houzelstein, D., et al. 2013. J Histochem Cytochem. 61: 348-61. PMID: 23360694
8. Metastatic Progression of Prostate Cancer Is Mediated by Autonomous Binding of Galectin-4-O-Glycan to Cancer Cells.  |  Tsai, CH., et al. 2016. Cancer Res. 76: 5756-5767. PMID: 27485450
9. Purification and characterization of the N-terminal domain of galectin-4 from rat small intestine.  |  Tardy, F., et al. 1995. FEBS Lett. 359: 169-72. PMID: 7867792
10. Two domains of rat galectin-4 bind to distinct structures of the intercellular borders of colorectal epithelia.  |  Wasano, K. and Hirakawa, Y. 1999. J Histochem Cytochem. 47: 75-82. PMID: 9857214

The preferred galectin-4 antibody is galectin-4 Antibody (E-2): sc-271209.