galectin-4 Antibody (E-2): sc-271209

galectin-4 Antibody (E-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects galectin-4 of mouse origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). galectin-4 (E-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Galectins, a family of soluble β-galactoside-binding lectins, play crucial roles in modulating cell-to-cell adhesion and interactions with the extracellular matrix, which are vital for processes such as tumor progression, pre-mRNA splicing, and apoptosis. Specifically, galectin-4, also referred to as Gal-4, L36, or LGALS4, is located on human chromosome 19q13.2 and consists of 323 amino acids, featuring two homologous carbohydrate recognition domains (CRDs) that are essential for binding activity. The structural integrity of these domains is critical, as they facilitate galectin-4′s ability to interact with glycoproteins and glycolipids, influencing cellular signaling pathways and adhesion processes. Notably, galectin-4 expression is associated with the malignant potential of human hepatocellular carcinoma and is modulated by factors such as cell-cell contact, serum growth factors, and the differentiation status of cells. In terms of localization, galectin-4 is predominantly expressed in the epithelial cells of the colon, rectum, and intestine, as well as in the HT29 and LS174T cell lines, where underexpression in colorectal cancer and preferential upregulation in cells susceptible to peritoneal dissemination highlight galectin-4′s potential as a biomarker for cancer progression.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

galectin-4 Antibody (E-2) References:

1. Identification and characterization of genes associated with human hepatocellular carcinogenesis.  |  Kondoh, N., et al. 1999. Cancer Res. 59: 4990-6. PMID: 10519413
2. Differential gene expression profiles of scirrhous gastric cancer cells with high metastatic potential to peritoneum or lymph nodes.  |  Hippo, Y., et al. 2001. Cancer Res. 61: 889-95. PMID: 11221876
3. Expression of endogenous galectin-1 and galectin-3 in intrahepatic cholangiocarcinoma.  |  Shimonishi, T., et al. 2001. Hum Pathol. 32: 302-10. PMID: 11274640
4. The role of galectin-4 in physiology and diseases.  |  Cao, ZQ. and Guo, XL. 2016. Protein Cell. 7: 314-24. PMID: 27017379
5. Molecular cloning, characterization, and expression of a human 14-kDa lectin.  |  Couraud, PO., et al. 1989. J Biol Chem. 264: 1310-6. PMID: 2910856
6. Normal Cortical Myelination in Galectin-4-Deficient Mice.  |  Brocca, ME., et al. 2022. Cells. 11: PMID: 36359880
7. Extracellular Galectin 4 Drives Immune Evasion and Promotes T-cell Apoptosis in Pancreatic Cancer.  |  Lidström, T., et al. 2023. Cancer Immunol Res. 11: 72-92. PMID: 36478037
8. Cytosolic galectin-4 enchains bacteria, restricts their motility, and promotes inflammasome activation in intestinal epithelial cells.  |  Li, CS., et al. 2023. Proc Natl Acad Sci U S A. 120: e2207091120. PMID: 36689650
9. Plasma Galectin-4 Levels Are Increased after Stroke in Mice and Humans.  |  Jujic, A., et al. 2023. Int J Mol Sci. 24: PMID: 37373212
10. An adherens junction protein is a member of the family of lactose-binding lectins.  |  Chiu, ML., et al. 1994. J Biol Chem. 269: 31770-6. PMID: 7989350
11. Cloning and expression of the mRNA of human galectin-4, an S-type lectin down-regulated in colorectal cancer.  |  Rechreche, H., et al. 1997. Eur J Biochem. 248: 225-30. PMID: 9310382
12. Galectin-4 and galectin-6 are two closely related lectins expressed in mouse gastrointestinal tract.  |  Gitt, MA., et al. 1998. J Biol Chem. 273: 2954-60. PMID: 9446608

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-galectin-4 Antibody (E-2) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-galectin-4 Antibody (E-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

galectin-4 Antibody (E-2) Specifications

Alternate Product Name: galectin-4

Product Clone Name: E-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 129-187

Epitope Target: galectin-4

Epitope Species: mouse

Product Reactivity/Detection: galectin-4

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for galectin-4 Antibody (E-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin