Anti-galectin-3 Antibody (A3A12) recommended for detection of galectin-3 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA

Anti-galectin-3 Antibody (A3A12): sc-53127

Anti-galectin-3 Antibody (A3A12) is rated 5.0 out of 5 by 10.
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Datasheets

    • galectin-3 Antibody (A3A12) is a mouse monoclonal IgG1 κ, cited in 14 publications, provided at 200 µg/ml
    • raised against recombinant full length human galectin-3
    • Anti-galectin-3 Antibody (A3A12) is recommended for detection of galectin-3 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA
    • Anti-galectin-3 Antibody (A3A12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of galectin-3 (A3A12): sc-53127.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for galectin-3 Antibody (A3A12) for WB and IHC(P) applications. These reagents are now offered in bundles with galectin-3 Antibody (A3A12) (see ordering information below).
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What is the dilution rate of galectin 3 antibody for IHC analysis?

Asked by: Anonymous
Thank you for your question. You can find this information in the antibody's datasheet: The recommended starting dilution is 1:50, with a dilution range of 1:50-1:500.
Answered by: Tech Support Europe
Date published: 2019-11-14

Would you be interested in providing a bulk order request for #sc-53127 in the amount of 150 mg? If so, could you also provide an estimated availability date? This is for my client who has an urgent need for this amount of mAb. Thanks!

Asked by: ActonBiotech
Please contact our Special Orders Department to inquire about availability and to receive a quote for a bulk quantity of this antibody. Please call our Technical Service Department, or email <[email protected]>.
Answered by: Technical Support
Date published: 2019-06-16

Can you recommend a positive control to use with galectin-3 (A3A12): sc-53127 monoclonal antibody for Western blot?

Asked by: jenniferc
We recommend HeLa (sc-2120), MCF7 (sc-2149), SW480 (sc-2155) or A-431 (sc-2122) nuclear extracts to use as positive controls as we have Western blot data showing specific detection of galectin-3 in these cell lines.
Answered by: Technical Support 15
Date published: 2017-01-19
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Rated 5 out of 5 by from single band The antibody I diluted at 1:1000, easy to get single band
Date published: 2017-09-05
Rated 5 out of 5 by from good signal for WB and IF I used this antibody for immunofluorescence (1:300) and WB(1:800), and I got very good and cleansignal.
Date published: 2017-05-27
Rated 5 out of 5 by from WB analysis It is a very good antibody. I performed for WB at 1:1000 and get very strong signal.
Date published: 2017-02-25
Rated 5 out of 5 by from Wow !! Superb Western blot and IHC data does not get better than this !!
Date published: 2017-02-01
Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of galectin-3 expression in HeLa, MCF7, SW480, WEHI-231 and A-431 nuclear extracts and Hs 294T and T98G whole cell lysates.
Date published: 2017-01-11
Rated 5 out of 5 by from Produced superb immunoperoxidase cytoplasmic Produced superb immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human small intestine tissue. -SCBT QC
Date published: 2015-05-01
Rated 5 out of 5 by from Published data for WB and IF in human renal Published data for WB and IF in human renal cancer samples (kidney). -SCBT Publication Review
Date published: 2015-04-09
Rated 5 out of 5 by from Using antibody for several years with no problems Using antibody for several years with no problems.
Date published: 2015-04-08
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