galectin-3 Antibody (B2C10): sc-32790

galectin-3 Antibody (B2C10) is a mouse monoclonal IgG1 kappa light chain antibody engineered to target galectin-3, also known as ac-2, hMac-2 (human Mac-2), GALBP (galactose-binding protein), CBP35 (carbohydrate-binding protein 35), and LGALS3 (lectin, galactoside-binding, soluble, 3). Anti-galectin-3 antibody (B2C10) is raised against recombinant human galectin-3 with an epitope mapped within the first 18 amino acids, and is recommended for detecting galectin-3 in mouse, rat, and human samples using western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Galectin-3 is a member of the galectin family of soluble β-galactoside-binding lectins characterized by a single carbohydrate recognition domain (CRD) that is essential for binding to galactose-containing glycoconjugates. This structural feature enables galectin-3 to mediate important biological processes such as cell-to-cell adhesion, cell-to-extracellular matrix (ECM) interactions, and cellular signaling pathways. The ability of galectin-3 to form oligomers and interact with multiple ligands through CRD allows influence over diverse cellular functions including tumor progression, metastasis, apoptosis, and pre-mRNA splicing. Galectin-3 can exist in both monomeric and oligomeric forms, which is important for regulating immune responses, inflammation, and cancer cell behavior. Galectin-3 functions both intracellularly, participating in signaling pathways and RNA processing, and extracellularly, facilitating cell adhesion and migration by interacting with ECM components. Galectin-3 expression is prominent in colonic and intestinal epithelial cells, inflammatory macrophages, papillary and follicular carcinomas, neoplastic astrocytes, and certain B and T lymphocytes. Upregulated galectin-3 expression closely associates with enhanced cancer progression and metastasis, thyroid malignancies, and Crohn′s disease, serving as a valuable diagnostic marker for conditions including Hurthle cell adenomas and carcinomas. Galectin-3 monoclonal antibody (B2C10) is available in non-conjugated form and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

galectin-3 Antibody (B2C10) References:

1. Identification of an internal gene to the human Galectin-3 gene with two different overlapping reading frames that do not encode Galectin-3.  |  Guittaut, M., et al. 2001. J Biol Chem. 276: 2652-7. PMID: 11160123
2. Expression of endogenous galectin-1 and galectin-3 in intrahepatic cholangiocarcinoma.  |  Shimonishi, T., et al. 2001. Hum Pathol. 32: 302-10. PMID: 11274640
3. Role of galectin-3 in adenocarcinoma liver metastasis.  |  Inufusa, H., et al. 2001. Int J Oncol. 19: 913-9. PMID: 11604988
4. Galectin-3 as a presurgical immunocytodiagnostic marker of minimally invasive follicular thyroid carcinoma.  |  Saggiorato, E., et al. 2001. J Clin Endocrinol Metab. 86: 5152-8. PMID: 11701669
5. Anti-Galectin-3 IgG autoantibodies in patients with Crohn's disease characterized by means of phage display peptide libraries.  |  Jensen-Jarolim, E., et al. 2001. J Clin Immunol. 21: 348-56. PMID: 11720007
6. Galectin-3 phosphorylation is required for its anti-apoptotic function and cell cycle arrest.  |  Yoshii, T., et al. 2002. J Biol Chem. 277: 6852-7. PMID: 11724777
7. Galectin-3 mediates the endocytosis of beta-1 integrins by breast carcinoma cells.  |  Furtak, V., et al. 2001. Biochem Biophys Res Commun. 289: 845-50. PMID: 11735123
8. Differential reactivity for galectin-3 in Hürthle cell adenomas and carcinomas.  |  Nascimento, MC., et al. 2001. Endocr Pathol. 12: 275-9. PMID: 11740048
9. Strikingly different localization of galectin-3 and galectin-4 in human colon adenocarcinoma T84 cells. Galectin-4 is localized at sites of cell adhesion.  |  Huflejt, ME., et al. 1997. J Biol Chem. 272: 14294-303. PMID: 9162064

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-galectin-3 Antibody (B2C10) has 104 citations in a variety of scientific publications.

Primary antibodies like Anti-galectin-3 Antibody (B2C10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

galectin-3 Antibody (B2C10) Specifications

Alternate Product Name: LGALS3

Product Clone Name: B2C10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-18

Epitope Target: Galectin-3

Epitope Species: human

Product Reactivity/Detection: galectin-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 104 citations in various scientific publications

Available Conjugates/Forms for galectin-3 Antibody (B2C10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, L: Azide-Free, PE: Phycoerythrin