Date published: 2026-5-5

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galectin-1 Antibody (B-2): sc-166617

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Datasheets
  • galectin-1 Antibody (B-2) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
  • raised against amino acids 1-45 mapping at the N-terminus of galectin-1 of human origin
  • recommended for detection of galectin-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • See galectin-1 (C-8): sc-166618 for galectin-1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for galectin-1 Antibody (B-2) for WB and IHC(P) applications. These reagents are now offered in bundles with galectin-1 Antibody (B-2) (see ordering information below).
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    Galectin-1 Antibody (B-2) is a mouse monoclonal IgG2a antibody that detects galectin-1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Galectin-1, also known as LGALS1, is a critical protein that plays a significant role in regulating cell proliferation, particularly in maintaining the G0 phase and controlling the transition through the G2 phase of the cell cycle. Anti-galectin-1 antibody (B-2) recognizes a protein consisting of 135 amino acids encoded by a single gene located on human chromosome 22q13.1. Galectin-1 features a single internal EcoRI site and a polyadenylation signal, which are important for stability and expression. Galectin-1 can be found in both intracellular and extracellular environments, highlighting versatile roles in various biological processes. Expression of galectin-1 occurs in several human tissues and cell lines, including placenta, lung, HL-6, HepG2, and CEM cells, underscoring importance in both normal physiology and disease states.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    galectin-1 Antibody (B-2)

    sc-166617
    200 µg/ml
    $322.00

    galectin-1 Antibody (B-2): m-IgG Fc BP-HRP Bundle

    sc-537512
    200 µg Ab; 10 µg BP
    $361.00

    galectin-1 Antibody (B-2): m-IgGκ BP-HRP Bundle

    sc-534796
    200 µg Ab; 40 µg BP
    $361.00

    galectin-1 Antibody (B-2): m-IgG2a BP-HRP Bundle

    sc-548038
    200 µg Ab; 10 µg BP
    $361.00

    Can I use anti-Galectin-1 and -3 for immunofluorescence staining without antigenic recovery? What dilution do you recommended?

    Asked by: Luz Marina Carrillo
    Thank you for your question. Yes, you should be OK to do IF with this antibody without antigen retrieval, as long as you are not using pfa or formalin to fix. If so, we would recommend antigen retrieval. Please contact our Technical Service Department by phone, e-mail or live chat if you have any further questions .
    Answered by: Tech Service
    Date published: 2017-08-28

    I am using galectin-1 (B-2): sc-166617 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

    Asked by: Randy McDonald
    We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-02-24
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    Rated 5 out of 5 by from Robust signal for WB in NIH/3T3 and KNRKRobust signal for WB in NIH/3T3 and KNRK whole cell lysates. -SCBT QC
    Date published: 2015-04-29
    Rated 5 out of 5 by from Produced excellent immunoperoxidase nuclearProduced excellent immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human ovary tissue. -SCBT QC
    Date published: 2015-03-11
    Rated 5 out of 5 by from LargeLarge, positive band in WB in HeLa whole cell lysate. -SCBT QC
    Date published: 2015-01-12
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    galectin-1 Antibody (B-2) is rated 5.0 out of 5 by 3.
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