Anti-galectin-1 Antibody (C-8) recommended for detection of galectin-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-galectin-1 Antibody (C-8): sc-166618

Anti-galectin-1 Antibody (C-8) is rated 5.0 out of 5 by 6.
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Datasheets

    • Anti-galectin-1 Antibody (C-8) is a mouse monoclonal IgG2a κ galectin-1 antibody, cited in 20 publications, provided at 200 µg/ml
    • raised against amino acids 1-45 mapping at the N-terminus of galectin-1 of human origin
    • Anti-galectin-1 Antibody (C-8) is recommended for detection of galectin-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-galectin-1 Antibody (C-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of galectin-1 (C-8): sc-166618.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for galectin-1 Antibody (C-8) for WB and IHC(P) applications. These reagents are now offered in bundles with galectin-1 Antibody (C-8) (see ordering information below).
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Dear Sirs, I am interesting for anti-galectin-1 antibody (raised in mouse, against human galectin-1) as detecting antibody for ELISA for human sera samples. Which one of your antibodies will you recommend for this purpose? Thanks in advance, Danica

Asked by: danicac
Thank you for your question. Yes, this antibody, galectin-1 (C-8) sc-166618, is a great choice for ELISA use. It is human reactive and will be a good choice for your application.
Answered by: Tech Service
Date published: 2019-10-02

I am thinking about using this antibody for a double stain. Do you have any suggestions for doing this with only mouse monoclonal antibodies?

Asked by: Cweed
We recommend using directly conjugated antibodies. This product, sc-166618, is available conjugated to FITC, AF488, and AF647.
Answered by: Technical Support 9
Date published: 2017-01-20
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Rated 5 out of 5 by from Clear and high signal I tested a sample and work really well with an ARPE-19 cell extract. Dil 1/200, ON incubation. Conjugated secondary antibody.
Date published: 2017-08-30
Rated 5 out of 5 by from Superb Very strong , clear western blot data; also great IHC staining
Date published: 2017-02-03
Rated 5 out of 5 by from Reliable and specific Choosing this antibody for galectin-1 studies is a smart choice. It gives nice strong bands in WB, and we see consistent specific staining in IHC.
Date published: 2016-12-16
Rated 5 out of 5 by from Exceptional immunoperoxidase nuclear and Exceptional immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human tonsil tissue. -SCBT QC
Date published: 2015-05-10
Rated 5 out of 5 by from Strong Strong, circular band seen in WB in HeLa, NIH/3T3 and KNRK whole cell lysates. -SCBT QC
Date published: 2015-03-13
Rated 5 out of 5 by from Publishable data for IHC(P) and WB in human Publishable data for IHC(P) and WB in human Pancreatic cancer tissue. -SCBT Publication Review
Date published: 2015-02-26
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