GAK Antibody (D-2): sc-137053

GAK Antibody (D-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects GAK protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-GAK antibody (D-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GAK protein, known as cyclin G associated kinase, plays a pivotal role in cell cycle regulation by interacting with cyclin G and cyclin-dependent kinases (Cdks). Specifically, GAK is crucial for the G1 to S phase transition, as GAK associates with cyclin G, which is known to be involved in the regulation of cell cycle progression. The interaction between GAK and cyclin G is significant because this interaction helps modulate the activity of Cdks, thereby influencing the timing of cell division and ensuring proper cellular function. Additionally, GAK has been shown to co-immunoprecipitate with Cdk5, further highlighting GAK′s importance in cell cycle control and signaling pathways. GAK′s ability to bind directly to cyclin G and involvement in phosphorylation processes underscores GAK′s relevance in cancer research and therapeutic development, making GAK (D-2) monoclonal antibody an essential tool for studying cell cycle dynamics and related disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

GAK Antibody (D-2) References:

1. D type cyclins associate with multiple protein kinases and the DNA replication and repair factor PCNA.  |  Xiong, Y., et al. 1992. Cell. 71: 505-14. PMID: 1358458
2. Identification and properties of an atypical catalytic subunit (p34PSK-J3/cdk4) for mammalian D type G1 cyclins.  |  Matsushime, H., et al. 1992. Cell. 71: 323-34. PMID: 1423597
3. Evidence that the G1-S and G2-M transitions are controlled by different cdc2 proteins in higher eukaryotes.  |  Fang, F. and Newport, JW. 1991. Cell. 66: 731-42. PMID: 1652371
4. Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family.  |  Koff, A., et al. 1991. Cell. 66: 1217-28. PMID: 1833068
5. Cyclin A is required for the onset of DNA replication in mammalian fibroblasts.  |  Girard, F., et al. 1991. Cell. 67: 1169-79. PMID: 1836977
6. Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B.  |  Pines, J. and Hunter, T. 1990. Nature. 346: 760-3. PMID: 2143810
7. Quantitative assessment of the association between GAK rs1564282 C/T polymorphism and the risk of Parkinson's disease.  |  Ma, ZG., et al. 2015. J Clin Neurosci. 22: 1077-80. PMID: 25975492
8. GAK and PRKCD are positive regulators of PRKN-independent mitophagy.  |  Munson, MJ., et al. 2021. Nat Commun. 12: 6101. PMID: 34671015
9. GAK and PRKCD kinases regulate basal mitophagy.  |  Munson, MJ., et al. 2022. Autophagy. 18: 467-469. PMID: 35001811
10. Cyclin-G-associated kinase GAK/dAux regulates autophagy initiation via ULK1/Atg1 in glia.  |  Zhang, S., et al. 2023. Proc Natl Acad Sci U S A. 120: e2301002120. PMID: 37428930
11. Cyclin G: a new mammalian cyclin with homology to fission yeast Cig1.  |  Tamura, K., et al. 1993. Oncogene. 8: 2113-8. PMID: 8336937
12. GAK: a cyclin G associated kinase contains a tensin/auxilin-like domain.  |  Kanaoka, Y., et al. 1997. FEBS Lett. 402: 73-80. PMID: 9013862

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GAK Antibody (D-2) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-GAK Antibody (D-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GAK Antibody (D-2) Specifications

Alternate Product Name: GAK

Product Clone Name: D-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-360

Epitope Target: GAK

Epitope Species: rat

Product Reactivity/Detection: GAK

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for GAK Antibody (D-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin