GADD 45γ Antibody (B-1): sc-393261

GADD 45γ Antibody (B-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects GADD 45γ in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GADD 45γ Antibody (B-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GADD 45γ plays a crucial role in cellular response to DNA damage, particularly in cell cycle regulation. GADD 45γ is primarily located in the nucleus, where GADD 45γ interacts with key proteins involved in DNA repair and cell cycle regulation. Nuclear localization of GADD 45γ enables timely activation of repair mechanisms, ensuring genomic stability. GADD 45γ binds to cyclin-dependent kinases (Cdks) and proliferating cell nuclear antigen (PCNA), facilitating DNA excision repair and inhibiting cell progression into S phase until DNA damage resolves. This function links p53-dependent cell cycle checkpoint to DNA repair processes, preventing damaged DNA propagation and contributing to tumor suppression. GADD 45-like proteins, including GADD 45β and GADD 45γ, respond to environmental stresses, highlighting their role in cellular stress responses and potential implications in cancer biology.

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GADD 45γ Antibody (B-1) References:

1. Wild-type p53 is a cell cycle checkpoint determinant following irradiation.  |  Kuerbitz, SJ., et al. 1992. Proc Natl Acad Sci U S A. 89: 7491-5. PMID: 1323840
2. Creative blocks: cell-cycle checkpoints and feedback controls.  |  Murray, AW. 1992. Nature. 359: 599-604. PMID: 1406993
3. A mammalian cell cycle checkpoint pathway utilizing p53 and GADD45 is defective in ataxia-telangiectasia.  |  Kastan, MB., et al. 1992. Cell. 71: 587-97. PMID: 1423616
4. GADD45γ regulates the thermogenic capacity of brown adipose tissue.  |  Gantner, ML., et al. 2014. Proc Natl Acad Sci U S A. 111: 11870-5. PMID: 25071184
5. Gadd45γ regulates cardiomyocyte death and post-myocardial infarction left ventricular remodelling.  |  Lucas, A., et al. 2015. Cardiovasc Res. 108: 254-67. PMID: 26370247
6. Mimicking Age-Associated Gadd45γ Dysregulation Results in Memory Impairments in Young Adult Mice.  |  Brito, DVC., et al. 2020. J Neurosci. 40: 1197-1210. PMID: 31826946
7. Modeling human age-associated increase in Gadd45γ expression leads to spatial recognition memory impairments in young adult mice.  |  Brito, DVC., et al. 2020. Neurobiol Aging. 94: 281-286. PMID: 32711258
8. Azacitidine regulates DNA methylation of GADD45γ in myelodysplastic syndromes.  |  Yang, Y., et al. 2021. J Clin Lab Anal. 35: e23597. PMID: 33080073
9. Gadd45γ alleviates collagen-induced arthritis by increasing IL-10 level and suppressing JNK activity.  |  Ma, Y., et al. 2025. Int Immunopharmacol. 151: 114329. PMID: 40007379
10. New link found between p53 and DNA repair.  |  Marx, J. 1994. Science. 266: 1321-2. PMID: 7973719
11. Interaction of the p53-regulated protein Gadd45 with proliferating cell nuclear antigen.  |  Smith, ML., et al. 1994. Science. 266: 1376-80. PMID: 7973727
12. A family of stress-inducible GADD45-like proteins mediate activation of the stress-responsive MTK1/MEKK4 MAPKKK.  |  Takekawa, M. and Saito, H. 1998. Cell. 95: 521-30. PMID: 9827804

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GADD 45γ Antibody (B-1) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-GADD 45γ Antibody (B-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GADD 45γ Antibody (B-1) Specifications

Alternate Product Name: GADD 45 gamma

Product Clone Name: B-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 95-159

Epitope Target: GADD 45γ

Epitope Species: human

Product Reactivity/Detection: GADD 45γ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for GADD 45γ Antibody (B-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin