GADD 45α Antibody (4T-27): sc-796
- GADD 45α Antibody (4T-27) is a mouse monoclonal IgG1 κ, cited in 31 publications, provided at 200 µg/ml
- raised against amino acids 1-165 representing full length GADD 45α of human origin
- Anti-GADD 45 alpha Antibody (4T-27) is recommended for detection of GADD 45α of mouse, rat and human origin by WB, IP and ELISA
- Anti-GADD 45 alpha Antibody (4T-27) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GADD 45α Antibody (4T-27) for WB applications. These reagents are now offered in bundles with GADD 45α Antibody (4T-27) (see ordering information below).
QUICK LINKS
GADD 45α Antibody (4T-27) is a mouse monoclonal IgG1 kappa light chain antibody that detects GADD 45α in mouse, rat, and human samples through western blotting, immunoprecipitation, and enzyme-linked immunosorbent assay. GADD 45α monoclonal antibody (4T-27) is offered in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. GADD 45α plays a crucial role in cellular response to DNA damage, particularly in regulating cell cycle checkpoints. When DNA damage occurs, cell cycle can be halted at G1 and G2 checkpoints, allowing for necessary repair processes before proceeding to DNA synthesis and mitosis. Tumor suppressor protein p53 enhances GADD 45α and p21 expression, with p21 inhibiting cyclin-dependent kinase (Cdk) activity to suppress cell proliferation. GADD 45α interacts with Cdks and proliferating cell nuclear antigen (PCNA), a key player in DNA replication and repair. GADD 45α enhances DNA excision repair in vitro and prevents cells from entering S phase, suggesting a critical link between p53-mediated cell cycle regulation and DNA repair mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GADD 45α Antibody (4T-27) | sc-796 | 200 µg/ml | $322.00 | |||
GADD 45α Antibody (4T-27): m-IgG Fc BP-HRP Bundle | sc-528119 | 200 µg Ab; 10 µg BP | $361.00 | |||
GADD 45α Antibody (4T-27): m-IgGκ BP-HRP Bundle | sc-520422 | 200 µg Ab, 40 µg BP | $361.00 | |||
GADD 45α Antibody (4T-27): m-IgG1 BP-HRP Bundle | sc-542771 | 200 µg Ab; 20 µg BP | $361.00 | |||
GADD 45α Antibody (4T-27) AC | sc-796 AC | 500 µg/ml, 25% agarose | $424.00 | |||
GADD 45α Antibody (4T-27) HRP | sc-796 HRP | 200 µg/ml | $322.00 | |||
GADD 45α Antibody (4T-27) FITC | sc-796 FITC | 200 µg/ml | $336.00 | |||
GADD 45α Antibody (4T-27) PE | sc-796 PE | 200 µg/ml | $349.00 | |||
GADD 45α Antibody (4T-27) Alexa Fluor® 488 | sc-796 AF488 | 200 µg/ml | $364.00 | |||
GADD 45α Antibody (4T-27) Alexa Fluor® 546 | sc-796 AF546 | 200 µg/ml | $364.00 | |||
GADD 45α Antibody (4T-27) Alexa Fluor® 594 | sc-796 AF594 | 200 µg/ml | $364.00 | |||
GADD 45α Antibody (4T-27) Alexa Fluor® 647 | sc-796 AF647 | 200 µg/ml | $364.00 | |||
GADD 45α Antibody (4T-27) Alexa Fluor® 680 | sc-796 AF680 | 200 µg/ml | $364.00 | |||
GADD 45α Antibody (4T-27) Alexa Fluor® 790 | sc-796 AF790 | 200 µg/ml | $364.00 |