DDIT3/GADD153/CHOP Antibody (H-5): sc-166682

GADD 153 Antibody (H-5) is a mouse monoclonal IgG3 kappa light chain antibody engineered to detect GADD 153, also known as CHOP or DDIT3, across multiple species including mouse, rat, human, equine, canine, bovine, and porcine using western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) techniques. GADD 153 monoclonal antibody (H-5) is raised against a peptide mapping at the C-terminus of human GADD153, ensuring precise and reliable binding for various experimental applications. GADD 153 (H-5) antibody recognizes a critical C/EBP-related nuclear transcription factor that plays a pivotal role in cellular stress responses, particularly in endoplasmic reticulum (ER) stress and unfolded protein response (UPR). Structurally, GADD 153 contains a basic leucine zipper (bZIP) domain that facilitates dimerization with other transcription factors such as ATF4 and members of the C/EBP family, enabling binding to specific DNA sequences and regulation of gene expression. These interactions are essential for promoting apoptosis and cell cycle arrest under prolonged stress conditions by modulating the expression of genes involved in these pathways. GADD 153 also interacts with proteins like Bcl-2 and Bim, which are key regulators of apoptotic machinery, thereby integrating stress signals to execute programmed cell death when necessary. Anti-GADD 153 antibody (H-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, to accommodate diverse experimental requirements.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

DDIT3/GADD153/CHOP Antibody (H-5) References:

1. Regulated translation initiation controls stress-induced gene expression in mammalian cells.  |  Harding, HP., et al. 2000. Mol Cell. 6: 1099-108. PMID: 11106749
2. Understanding mesenchymal cancer: the liposarcoma-associated FUS-DDIT3 fusion gene as a model.  |  Pérez-Mancera, PA. and Sánchez-García, I. 2005. Semin Cancer Biol. 15: 206-14. PMID: 15826835
3. Inhibition of glioma growth by flavokawain B is mediated through endoplasmic reticulum stress induced autophagy.  |  Wang, J., et al. 2018. Autophagy. 14: 2007-2022. PMID: 30025493
4. DDIT3 (CHOP) contributes to retinal ganglion cell somal loss but not axonal degeneration in DBA/2J mice.  |  Marola, OJ., et al. 2019. Cell Death Discov. 5: 140. PMID: 31632741
5. DDIT3 antagonizes innate immune response to promote bovine alphaherpesvirus 1 replication via the DDIT3-SQSTM1-STING pathway.  |  Wang, S., et al. 2022. Virulence. 13: 514-529. PMID: 35259065
6. The FUS::DDIT3 fusion oncoprotein inhibits BAF complex targeting and activity in myxoid liposarcoma.  |  Zullow, HJ., et al. 2022. Mol Cell. 82: 1737-1750.e8. PMID: 35390276
7. A novel DDIT3 activator dehydroevodiamine effectively inhibits tumor growth and tumor cell stemness in pancreatic cancer.  |  Zhu, SL., et al. 2024. Phytomedicine. 128: 155377. PMID: 38503154
8. DDIT3 is associated with breast cancer prognosis and immune microenvironment: an integrative bioinformatic and immunohistochemical analysis.  |  Yu, X., et al. 2024. J Cancer. 15: 3873-3889. PMID: 38911383
9. Transcription factor DDIT3 is a potential driver in pancreatic cancer.  |  Ju, G., et al. 2024. Cell Mol Biol (Noisy-le-grand). 70: 148-154. PMID: 39097882
10. DDIT3 switches osteogenic potential of BMP9 to lipogenic by attenuating Wnt/β-catenin signaling via up-regulating DKK1 in mesenchymal stem cells.  |  Luo, HH., et al. 2024. Aging (Albany NY). 16: 12543-12558. PMID: 39331002

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DDIT3/GADD153/CHOP Antibody (H-5) has 30 citations in a variety of scientific publications.

Primary antibodies like Anti-DDIT3/GADD153/CHOP Antibody (H-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DDIT3/GADD153/CHOP Antibody (H-5) Specifications

Alternate Product Name: GADD 153

Product Clone Name: H-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 149-168

Epitope Target: GADD 153

Epitope Species: human

Product Reactivity/Detection: GADD 153

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 30 citations in various scientific publications

Available Conjugates/Forms for DDIT3/GADD153/CHOP Antibody (H-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred DDIT3/GADD153/CHOP antibody is DDIT3/GADD153/CHOP Antibody (B-3): sc-7351.